Construct: ORF TRCN0000470967
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016497.1_s317c1
- Derived from:
- ccsbBroadEn_09181
- DNA Barcode:
- AAACATAAGTACGGTTAACTCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMSS1 (84319)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470967
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84319 | CMSS1 | cms1 ribosomal small subuni... | NM_032359.4 | 99.8% | 99.6% | 413A>G |
| 2 | human | 84319 | CMSS1 | cms1 ribosomal small subuni... | NM_001167924.1 | 92.7% | 92.1% | (many diffs) |
| 3 | mouse | 66497 | Cmss1 | cms small ribosomal subunit 1 | NM_025599.3 | 84.7% | 82.1% | (many diffs) |
| 4 | mouse | 66497 | Cmss1 | cms small ribosomal subunit 1 | XM_006522460.2 | 74.4% | 72.8% | (many diffs) |
| 5 | mouse | 66497 | Cmss1 | cms small ribosomal subunit 1 | XM_011245995.2 | 74.4% | 72.8% | (many diffs) |
| 6 | mouse | 66497 | Cmss1 | cms small ribosomal subunit 1 | XM_017317115.1 | 74.4% | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agacgatctc ggagacgagt ggtgggagaa ccagccgact ggagcaggca 121 gcagcccaga agcatcagat ggtgaaggag aaggagacac agaagtgatg cagcaggaga 181 cagttccagt tcctgtacct tcagagaaaa ccaaacagcc taaagaatgt tttttgatac 241 aaccaaagga aagaaaagag aataccacca agaccaggaa aagaagaaag aagaaaatta 301 ctgatgttct tgcaaaatca gaaccaaaac cagggttacc tgaagaccta cagaagctga 361 tgaaggacta ttatagcagc agacgcttgg tgattgaatt agaagaactg aacctgccag 421 actcctgttt cctcaaggcc aatgatttga ctcacagtct ttcctcatac ctaaaaggaa 481 tttgtcctaa gtgggtaaaa cttaggaaga accacagtga gaagaaatcg gtcctgatgc 541 tgatcatctg cagctcggcc gtccgagccc tggagctcat taggtcgatg acagcattca 601 gaggagacgg caaagttata aaattatttg caaagcacat aaaggtccaG GCGCAGGTAA 661 AGTTGCTGGA GAAGCGTGTG GTGCACCTGG GTGTAGGAAC TCCGGGGAGA ATTAAAGAAC 721 TTGTTAAACA AGGTGGCCTT AATTTGAGCC CCTTAAAATT TCTGGTTTTT GACTGGAACT 781 GGAGAGATCA GAAGTTGAGG AGAATGATGG ACATTCCCGA GATAAGAAAG GAGGTATTCG 841 AACTTCTGGA AATGGGAGTG CTCAGTCTGT GCAAGTCAGA ATCCTTGAAA CTGGGCCTTT 901 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAAA ACATAAGTAC GGTTAACTCG CAACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt