Construct: ORF TRCN0000470971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017989.1_s317c1
- Derived from:
- ccsbBroadEn_00608
- DNA Barcode:
- GAAAATGGTGAAGAGCAAACAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAD1 (2571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2571 | GAD1 | glutamate decarboxylase 1 | NM_013445.3 | 100% | 100% | |
| 2 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_005246444.3 | 100% | 100% | |
| 3 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_017003758.2 | 100% | 100% | |
| 4 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_017003757.2 | 56% | 54.1% | (many diffs) |
| 5 | human | 2571 | GAD1 | glutamate decarboxylase 1 | NM_000817.3 | 37.2% | 36% | (many diffs) |
| 6 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_011510922.1 | 37.2% | 36% | (many diffs) |
| 7 | human | 2571 | GAD1 | glutamate decarboxylase 1 | XM_017003756.1 | 37.2% | 36% | (many diffs) |
| 8 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | NM_001312900.1 | 90.4% | 96.8% | (many diffs) |
| 9 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | NM_008077.5 | 33.3% | 34.8% | (many diffs) |
| 10 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_006498765.3 | 33.3% | 34.8% | (many diffs) |
| 11 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_006498766.3 | 33.3% | 34.8% | (many diffs) |
| 12 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_017315594.1 | 31.8% | 33.2% | (many diffs) |
| 13 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_017315597.1 | 31.8% | 33.2% | (many diffs) |
| 14 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XM_017315599.1 | 31.8% | 33.2% | (many diffs) |
| 15 | mouse | 14415 | Gad1 | glutamate decarboxylase 1 | XR_001780815.1 | 16.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcttcgacc ccatcttcgt ccgcaacctc ctcgaacgcg ggagcggacc 121 ccaataccac taacctgcgc cccacaacgt acgatacctg gtgcggcgtg gcccatggat 181 gcaccagaaa actggggctc aagatctgcg gcttcttgca aaggaccaac agcctggaag 241 agaagagtcg ccttgtgagt gccttcaagg agaggcaatc ctccaagaac cTGCTTTCCT 301 GTGAAAACAG CGACCGGGAT GCCCGCTTCC GGCGCACAGA GACTGACTTC TCTAATCTGT 361 TTGCTAGAGA TCTGCTTCCG GCTAAGAACG GTGAGGAGCA AACCGTGCAA TTCCTCCTGG 421 AAGTGGTGGA CATACTCCTC AACTATGTCC GCAAGACATT TGATCGCTCC ACCAAGGTGC 481 TGGACTTTCA TCACCCACAC CAGTTGCTGG AAGGCATGGA GGGCTTCAAC TTGGAGCTCT 541 CTGACCACCC CGAGTCCCTG GAGCAGATCC TGGTTGACTG CAGAGACACC TTGAAGTATG 601 GGGTTCGCAC AGGTCATCCT CGATTTTTCA ACCAGCTCTC CACTGGATTG GATATTATTG 661 GCCTAGCTGG AGAATGGCTG ACATCAACGG CCAATACCAA CATGCCATCA GACATGAGGG 721 AGTGTTGGTT GCTACGGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAAAATG GTGAAGAGCA 901 AACAACAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt