Construct: ORF TRCN0000470979
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002048.3_s317c1
- Derived from:
- ccsbBroadEn_14830
- DNA Barcode:
- ACTGACTTTTCCCCTTGCATTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA1 (6195)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470979
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_002953.4 | 99.9% | 100% | 51T>G;507T>C |
| 2 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_001330441.2 | 96.2% | 95.9% | (many diffs) |
| 3 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | NM_001006665.2 | 95.8% | 95.3% | (many diffs) |
| 4 | human | 6195 | RPS6KA1 | ribosomal protein S6 kinase A1 | XM_024448871.1 | 87.4% | 87.4% | 0_1ins276;231T>C |
| 5 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_009097.5 | 91% | 98.5% | (many diffs) |
| 6 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_001285505.1 | 89.5% | 97% | (many diffs) |
| 7 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | XM_006538677.3 | 87.4% | 92.9% | (many diffs) |
| 8 | mouse | 20111 | Rps6ka1 | ribosomal protein S6 kinase... | NM_001285506.1 | 86.8% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2271
- ORF length:
- 2205
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gctcgcccag ctcaaggagc cctggccgct catggagcta gtgccgctgg 121 acccggagaa tggacagacc tcaggggaag aagctggact tcagccgtcc aaggatgagg 181 gcgtcctcaa ggagatctcc atcacgcacc acgtcaaggc tggctctgag aaggctgatc 241 catcccattt cgagctcctc aaggttctgg gccagggatc ctttggcaaa gtcttcctgg 301 tgcggaaagt cacccggcct gacagtgggc acctgtatgc tatgaaggtg ctgaagaagg 361 caacgctgaa agtacgtgac cgcgtccgga ccaagatgga gagagacatc ctggctgatg 421 taaatcaccc attcgtggtg aagctgcact atgccttcca gaccgagggc aagctctatc 481 tcattctgga cttcctgcgt ggtggggacc tcttcacccg gctctcaaaa gaggtgatgt 541 tcacggagga ggatgtgaag ttttacctgg ccgagctggc tctgggcctg gatcacctgc 601 acagcctggg tatcatttac agagacctca agcctgagaa catccttctg gatgaggagg 661 gccacatcaa actcactgac tttggcctga gcaaagaggc cattgaccac gagaagaagg 721 cctattcttt ctgcgggaca gtggagtaca tggcccctga ggtcgtcaac cgccagggcc 781 actcccatag tgcggactgg tggtcctatg gggtgttgat gtttgagatg ctgacgggct 841 ccctgccctt ccaggggaag gaccggaagg agaccatgac actgattctg aaggcgaagc 901 taggcatgcc ccagtttctg agcactgaag cccagagcct cttgcgggcc ctgttcaagc 961 ggaatcctgc caaccggctc ggctccggcc ctgatggggc agaggaaatc aagcggcatg 1021 tcttctactc caccattgac tggaataagc tataccgtcg tgagatcaag ccacccttca 1081 agccagcagt ggctcagcct gatgacacct tctactttga caccgagttc acgtcccgca 1141 cacccaagga ttccccaggc atccccccca gcgctggggc ccatcagctg ttccggggct 1201 tcagcttcgt ggccaccggc ctgatggaag acgacggcaa gcctcgtgcc ccgcaggcac 1261 ccctgcactc ggtggtacag caactccatg ggaagaacct ggtttttagt gacggctacg 1321 tggtaaagga gacaattggt gtgggctcct actctgagtg caagcgctgt gtccacaagg 1381 ccaccaacat ggagtatgct gtcaaggtca ttgataagag caagcgggat ccttcagaag 1441 agattgagat tcttctgcgg tatggccagc accccaacat catcactctg aaagatgtgt 1501 atgatgatgg caaacacgtg tacctggtga cagagctgat gcggggtggg gagctgctgg 1561 acaagatcct gcggcagaag ttcttctcag agcgggaggc cagctttgtc ctgcacacca 1621 ttggcaaaac tgtggagtat ctgcactcac agggggttgt gcacagggac ctgaagccca 1681 gcaacatcct gtatgtggac gagtccggga atcccgagtg cctgcgcatc tgtgactttg 1741 gttttgccaa acagctgcgg gctgagaatg ggctcctcat gacaccttgc tacacagcca 1801 actttgtggc gcctgaggtg ctgaagcgcc agggctacga tgaaggctgc gacatcTGGA 1861 GCCTGGGCAT TCTGCTGTAC ACCATGCTGG CAGGATATAC TCCATTTGCC AACGGTCCCA 1921 GTGACACACC AGAGGAAATC CTAACCCGGA TCGGCAGTGG GAAGTTTACC CTCAGTGGGG 1981 GAAATTGGAA CACAGTTTCA GAGACAGCCA AGGACCTGGT GTCCAAGATG CTACACGTGG 2041 ATCCCCACCA GCGCCTCACA GCTAAGCAGG TTCTGCAGCA TCCATGGGTC ACCCAGAAAG 2101 ACAAGCTTCC CCAAAGCCAG CTGTCCCACC AGGACCTACA GCTTGTGAAG GGAGCCATGG 2161 CTGCCACGTA CTCCGCACTC AACAGCTCCA AGCCCACCCC CCAGCTGAAG CCCATCGAGT 2221 CATCCATCCT GGCCCAGCGG CGAGTGAGGA AGTTGCCATC CACCACCCTG TGCCCAACTT 2281 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2341 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2401 CTTGTGGAAA GGACGAACTG ACTTTTCCCC TTGCATTGCC ACGCGTTAAG TCgacaatca 2461 acctctggat tacaaaattt gtgaaagatt