Construct: ORF TRCN0000470982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016609.4_s317c1
- Derived from:
- ccsbBroadEn_14551
- DNA Barcode:
- TGCGCCGTGGTGTACAAAAACGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMPR2 (659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470982
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 659 | BMPR2 | bone morphogenetic protein ... | NM_001204.7 | 100% | 100% | |
| 2 | human | 659 | BMPR2 | bone morphogenetic protein ... | XM_011511687.1 | 99.9% | 99.8% | 2866_2867insTAG |
| 3 | mouse | 12168 | Bmpr2 | bone morphogenetic protein ... | NM_007561.4 | 91.6% | 96.6% | (many diffs) |
| 4 | mouse | 12168 | Bmpr2 | bone morphogenetic protein ... | XM_006495633.1 | 91.5% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3180
- ORF length:
- 3114
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ttcctcgctg cagcggccct ggcgggtgcc ctggctacca tggaccatcc 121 tgctggtcag cactgcggct gcttcgcaga atcaagaacg gctatgtgcg tttaaagatc 181 cgtatcagca agaccttggg ataggtgaga gtagaatctc tcatgaaaat gggacaatat 241 tatgctcgaa aggtagcacc tgctatggcc tttgggagaa atcaaaaggg gacataaatc 301 ttgtaaaaca aggatgttgg tctcacattg gagatcccca agagtgtcac tatgaagaat 361 gtgtagtaac taccactcct ccctcaattc agaatggaac ataccgtttc tgctgttgta 421 gcacagattt atgtaatgtc aactttactg agaattttcc acctcctgac acaacaccac 481 tcagtccacc tcattcattt aaccgagatg agacaataat cattgctttg gcatcagtct 541 ctgtattagc tgttttgata gttgccttat gctttggata cagaatgttg acaggagacc 601 gtaaacaagg tcttcacagt atgaacatga tggaggcagc agcatccgaa ccctctcttg 661 atctagataa tctgaaactg ttggagctga ttggccgagg tcgatatgga gcagtatata 721 aaggctcctt ggatgagcgt ccagttgctg taaaagtgtt ttcctttgca aaccgtcaga 781 attttatcaa cgaaaagaac atttacagag tgcctttgat ggaacatgac aacattgccc 841 gctttatagt tggagatgag agagtcactg cagatggacg catggaatat ttgcttgtga 901 tggagtacta tcccaatgga tctttatgca agtatttaag tctccacaca agtgactggg 961 taagctcttg ccgtcttgct cattctgtta ctagaggact ggcttatctt cacacagaat 1021 taccacgagg agatcattat aaacctgcaa tttcccatcg agatttaaac agcagaaatg 1081 tcctagtgaa aaatgatgga acctgtgtta ttagtgactt tggactgtcc atgaggctga 1141 ctggaaatag actggtgcgc ccaggggagg aagataatgc agccataagc gaggttggca 1201 ctatcagata tatggcacca gaagtgctag aaggagctgt gaacttgagg gactgtgaat 1261 cagctttgaa acaagtagac atgtatgctc ttggactaat ctattgggag atatttatga 1321 gatgtacaga cctcttccca ggggaatccg taccagagta ccagatggct tttcagacag 1381 aggttggaaa ccatcccact tttgaggata tgcaggttct cgtgtctagg gaaaaacaga 1441 gacccaagtt cccagaagcc tggaaagaaa atagcctggc agtgaggtca ctcaaggaga 1501 caatcgaaga ctgttgggac caggatgcag aggctcggct tactgcacag tgtgctgagg 1561 aaaggatggc tgaacttatg atgatttggg aaagaaacaa atctgtgagc ccaacagtca 1621 atccaatgtc tactgctatg cagaatgaac gcaacctgtc acataatagg cgtgtgccaa 1681 aaattggtcc ttatccagat tattcttcct cctcatacat tgaagactct atccatcata 1741 ctgacagcat cgtgaagaat atttcctctg agcattctat gtccagcaca cctttgacta 1801 taggggaaaa aaaccgaaat tcaattaact atgaacgaca gcaagcacaa gctcgaatcc 1861 ccagccctga aacaagtgtc accagcctct ccaccaacac aacaaccaca aacaccacag 1921 gactcacgcc aagtactggc atgactacta tatctgagat gccataccca gatgaaacaa 1981 atctgcatac cacaaatgtt gcacagtcaa ttgggccaac ccctgtctgc ttacagctga 2041 cagaagaaga cttggaaacc aacaagctag acccaaaaga agttgataag aacctcaagg 2101 aaagctctga tgagaatctc atggagcact ctcttaaaca gttcagtggc ccagacccac 2161 tgagcagtac tagttctagc ttgctttacc cactcataaa acttgcagta gaagcaactg 2221 gacagcagga cttcacacag actgcaaatg gccaagcatg tttgattcct gatgttctgc 2281 ctactcagat ctatcctctc cccaagcagc agaaccttcc caagagacct actagtttgc 2341 ctttgaacac caaaaattca acaaaagagc cccggctaaa atttggcagc aagcacaaat 2401 caaacttgaa acaagtcgaa actggagttg ccaagatgaa tacaatcaat gcagcagaac 2461 ctcatgtggt gacagtcacc atgaatggtg tggcaggtag aaaccacagt gttaactccc 2521 atgctgccac aacccaatat gccaatggga cagtactatc tggccaaaca accaacatag 2581 tgacacatag ggcccaagaa atgttgcaga atcagtttat tggtgaggac acccggctga 2641 atattaattc cagtcctgat gagcatgagc ctttactgag acgagagcaa caagctggcc 2701 atgatgaagg tgttctggat cgtcttgtgg acaggaggga acggccacta gaaggtggcc 2761 gaactaattc caataacaac aacagcaatC CATGTTCAGA ACAAGATGTT CTTGCACAGG 2821 GTGTTCCAAG CACAGCAGCA GATCCTGGGC CATCAAAGCC CAGAAGAGCA CAGAGGCCTA 2881 ATTCTCTGGA TCTTTCAGCC ACAAATGTCC TGGATGGCAG CAGTATACAG ATAGGTGAGT 2941 CAACACAAGA TGGCAAATCA GGATCAGGTG AAAAGATCAA GAAACGTGTG AAAACTCCCT 3001 ATTCTCTTAA GCGGTGGCGC CCCTCCACCT GGGTCATCTC CACTGAATCG CTGGACTGTG 3061 AAGTCAACAA TAATGGCAGT AACAGGGCAG TTCATTCCAA ATCCAGCACT GCTGTTTACC 3121 TTGCAGAAGG AGGCACTGCT ACAACCATGG TGTCTAAAGA TATAGGAATG AACTGTCTGT 3181 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3241 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3301 TTTATATATC TTGTGGAAAG GACGATGCGC CGTGGTGTAC AAAAACGGAA CGCGTTAAGT 3361 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt