Transcript: Human NM_001204.7

Homo sapiens bone morphogenetic protein receptor type 2 (BMPR2), mRNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
BMPR2 (659)
Length:
12068
CDS:
1149..4265

Additional Resources:

NCBI RefSeq record:
NM_001204.7
NBCI Gene record:
BMPR2 (659)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144883 AGCAACTGGACGCTCATCCA pXPR_003 AGG 667 21% 6 1.1619 BMPR2 BMPR2 76391
2 BRDN0001144950 CCTTTGGGAGAAATCAAAAG pXPR_003 GGG 220 7% 2 0.1429 BMPR2 BMPR2 76390
3 BRDN0001146229 CAGCACACCTTTGACTATAG pXPR_003 GGG 1735 56% 12 0.0542 BMPR2 BMPR2 76389
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001204.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197218 GAACGGCTATGTGCGTTTAAA pLKO.1 1239 CDS 100% 15.000 21.000 N BMPR2 n/a
2 TRCN0000000456 GCCTATGGAGTGAAATTATTT pLKO.1 4277 3UTR 100% 15.000 21.000 N BMPR2 n/a
3 TRCN0000194669 CCGAACTAATTCCAATAACAA pLKO.1 3842 CDS 100% 5.625 7.875 N BMPR2 n/a
4 TRCN0000000460 CAATCCAATGTCTACTGCTAT pLKO.1 2702 CDS 100% 4.950 6.930 N BMPR2 n/a
5 TRCN0000000458 CGCAGAATCAAGAACGGCTAT pLKO.1 1228 CDS 100% 4.050 5.670 N BMPR2 n/a
6 TRCN0000218801 ATTACCACGAGGAGATCATTA pLKO_005 2102 CDS 100% 13.200 10.560 N Bmpr2 n/a
7 TRCN0000194724 CCCTCTCTTGATCTAGATAAT pLKO.1 1734 CDS 100% 13.200 9.240 N BMPR2 n/a
8 TRCN0000195156 CCTAACTGTATACCAGAATTA pLKO.1 7048 3UTR 100% 13.200 9.240 N BMPR2 n/a
9 TRCN0000196987 GCTACAACCATGGTGTCTAAA pLKO.1 4221 CDS 100% 13.200 9.240 N BMPR2 n/a
10 TRCN0000195509 CCCAAGAAATGTTGCAGAATC pLKO.1 3676 CDS 100% 10.800 7.560 N BMPR2 n/a
11 TRCN0000196253 GCTACCTGATTTCTTACTTTC pLKO.1 4924 3UTR 100% 10.800 7.560 N BMPR2 n/a
12 TRCN0000022529 GCCAAGATGAATACAATCAAT pLKO.1 3513 CDS 100% 5.625 3.938 N Bmpr2 n/a
13 TRCN0000000459 GCCGTCTTGCTCATTCTGTTA pLKO.1 2053 CDS 100% 4.950 3.465 N BMPR2 n/a
14 TRCN0000000457 GCCCGCTTTATAGTTGGAGAT pLKO.1 1920 CDS 100% 4.050 2.835 N BMPR2 n/a
15 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 9104 3UTR 100% 4.950 2.475 Y ERAP2 n/a
16 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 9105 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001204.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00169 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00169 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469790 TAATTGCCGGGGTACCCGGTCTAC pLX_317 14.1% 100% 100% V5 n/a
4 ccsbBroadEn_14551 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_14551 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000470982 TGCGCCGTGGTGTACAAAAACGGA pLX_317 12.7% 100% 100% V5 n/a
7 TRCN0000488108 GGGCTTCCCTTATTTTATGTAACA pLX_317 9.6% 99.9% 99.8% V5 (not translated due to prior stop codon) 67A>G;1590C>A n/a
8 TRCN0000487951 CCATATCACGCACGTGGACTCCCT pLX_317 7.1% 99.9% 99.7% V5 67A>G;1590C>A;3114_3115insG n/a
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