Construct: ORF TRCN0000470996

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004470.1_s317c1
Derived from:
ccsbBroadEn_08873
DNA Barcode:
GACGGTAAGCCAATCCCTTTATGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
P2RY12 (64805)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470996
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64805 P2RY12 purinergic receptor P2Y12 NM_022788.4 99.8% 100% 18C>T;36T>G
2 human 64805 P2RY12 purinergic receptor P2Y12 NM_176876.3 99.8% 100% 18C>T;36T>G
3 mouse 70839 P2ry12 purinergic receptor P2Y, G-... NM_027571.3 82.1% 86.2% (many diffs)
4 mouse 70839 P2ry12 purinergic receptor P2Y, G-... XM_006502083.3 82.1% 86.2% (many diffs)
5 mouse 70839 P2ry12 purinergic receptor P2Y, G-... XM_006502084.3 82.1% 86.2% (many diffs)
6 mouse 70839 P2ry12 purinergic receptor P2Y, G-... XM_006502085.3 82.1% 86.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1092
ORF length:
1026
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca agccgtcgac aatctcacct ctgcgcctgg gaacaccagt ctgtgcacca
121gagactacaa aatcacccag gtcctcttcc cactgctcta cactgtcctg ttttttgttg
181gacttatcac aaatggcctg gcgatgagga ttttctttca aatccggagt aaatcaaact
241ttattatttt tcttaagaac acagtcattt ctgatcttct catgattctg acttttccat
301tcaaaattct tagtgatgcc aaactgggaa caggaccact gagaactttt gtgtgtcaag
361ttacctccgt catattttat ttcacaatgt atatcagtat ttcattcctg ggactgataa
421ctatcgatcg ctaccagaag accaccaggc catttaaaac atccaacccc aaaaatctct
481tgggggctaa gattctctct gttgtcatct gggcattcat gttcttactc tctttgccta
541acatgattct gaccaacagg cagccgagag acaagaatgt gaagaaatgc tctttcctta
601aatcagagtt cggtctagtc tggcatgaaa tagtaaatta caTCTGTCAA GTCATTTTCT
661GGATTAATTT CTTAATTGTT ATTGTATGTT ATACACTCAT TACAAAAGAA CTGTACCGGT
721CATACGTAAG AACGAGGGGT GTAGGTAAAG TCCCCAGGAA AAAGGTGAAC GTCAAAGTTT
781TCATTATCAT TGCTGTATTC TTTATTTGTT TTGTTCCTTT CCATTTTGCC CGAATTCCTT
841ACACCCTGAG CCAAACCCGG GATGTCTTTG ACTGCACTGC TGAAAATACT CTGTTCTATG
901TGAAAGAGAG CACTCTGTGG TTAACTTCCT TAAATGCATG CCTGGATCCG TTCATCTATT
961TTTTCCTTTG CAAGTCCTTC AGAAATTCCT TGATAAGTAT GCTGAAGTGC CCCAATTCTG
1021CAACATCTCT GTCCCAGGAC AATAGGAAAA AAGAACAGGA TGGTGGTGAC CCAAATGAAG
1081AGACTCCAAT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1141ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1201CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAC GGTAAGCCAA TCCCTTTATG
1261GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)