Construct: ORF TRCN0000470996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004470.1_s317c1
- Derived from:
- ccsbBroadEn_08873
- DNA Barcode:
- GACGGTAAGCCAATCCCTTTATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P2RY12 (64805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64805 | P2RY12 | purinergic receptor P2Y12 | NM_022788.4 | 99.8% | 100% | 18C>T;36T>G |
| 2 | human | 64805 | P2RY12 | purinergic receptor P2Y12 | NM_176876.3 | 99.8% | 100% | 18C>T;36T>G |
| 3 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | NM_027571.3 | 82.1% | 86.2% | (many diffs) |
| 4 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502083.3 | 82.1% | 86.2% | (many diffs) |
| 5 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502084.3 | 82.1% | 86.2% | (many diffs) |
| 6 | mouse | 70839 | P2ry12 | purinergic receptor P2Y, G-... | XM_006502085.3 | 82.1% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1092
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca agccgtcgac aatctcacct ctgcgcctgg gaacaccagt ctgtgcacca 121 gagactacaa aatcacccag gtcctcttcc cactgctcta cactgtcctg ttttttgttg 181 gacttatcac aaatggcctg gcgatgagga ttttctttca aatccggagt aaatcaaact 241 ttattatttt tcttaagaac acagtcattt ctgatcttct catgattctg acttttccat 301 tcaaaattct tagtgatgcc aaactgggaa caggaccact gagaactttt gtgtgtcaag 361 ttacctccgt catattttat ttcacaatgt atatcagtat ttcattcctg ggactgataa 421 ctatcgatcg ctaccagaag accaccaggc catttaaaac atccaacccc aaaaatctct 481 tgggggctaa gattctctct gttgtcatct gggcattcat gttcttactc tctttgccta 541 acatgattct gaccaacagg cagccgagag acaagaatgt gaagaaatgc tctttcctta 601 aatcagagtt cggtctagtc tggcatgaaa tagtaaatta caTCTGTCAA GTCATTTTCT 661 GGATTAATTT CTTAATTGTT ATTGTATGTT ATACACTCAT TACAAAAGAA CTGTACCGGT 721 CATACGTAAG AACGAGGGGT GTAGGTAAAG TCCCCAGGAA AAAGGTGAAC GTCAAAGTTT 781 TCATTATCAT TGCTGTATTC TTTATTTGTT TTGTTCCTTT CCATTTTGCC CGAATTCCTT 841 ACACCCTGAG CCAAACCCGG GATGTCTTTG ACTGCACTGC TGAAAATACT CTGTTCTATG 901 TGAAAGAGAG CACTCTGTGG TTAACTTCCT TAAATGCATG CCTGGATCCG TTCATCTATT 961 TTTTCCTTTG CAAGTCCTTC AGAAATTCCT TGATAAGTAT GCTGAAGTGC CCCAATTCTG 1021 CAACATCTCT GTCCCAGGAC AATAGGAAAA AAGAACAGGA TGGTGGTGAC CCAAATGAAG 1081 AGACTCCAAT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAC GGTAAGCCAA TCCCTTTATG 1261 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t