Construct: ORF TRCN0000470997

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011681.1_s317c1
Derived from:
ccsbBroadEn_15404
DNA Barcode:
ATCGTCCTTTTTTCTTTTCATCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EEF1D (1936)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470997
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1936 EEF1D eukaryotic translation elon... NM_001130055.3 99.8% 100% 636C>T
2 human 1936 EEF1D eukaryotic translation elon... NM_001130057.3 99.8% 100% 636C>T
3 human 1936 EEF1D eukaryotic translation elon... NM_001289950.3 99.8% 100% 636C>T
4 human 1936 EEF1D eukaryotic translation elon... NM_001960.6 99.8% 100% 636C>T
5 human 1936 EEF1D eukaryotic translation elon... XM_006716525.1 99.8% 100% 636C>T
6 human 1936 EEF1D eukaryotic translation elon... NM_001195203.3 93.1% 93.2% 230_231ins57;579C>T
7 human 1936 EEF1D eukaryotic translation elon... NM_001130056.4 91.3% 91.4% 117_118ins72;564C>T
8 human 1936 EEF1D eukaryotic translation elon... NM_001317743.3 91.3% 91.4% 117_118ins72;564C>T
9 human 1936 EEF1D eukaryotic translation elon... NM_001330646.2 91.3% 91.4% 117_118ins72;564C>T
10 human 1936 EEF1D eukaryotic translation elon... XM_006716519.4 43.4% 43.4% 1_1095del;1731C>T
11 human 1936 EEF1D eukaryotic translation elon... XM_017013171.1 43.4% 43.4% 1_1095del;1731C>T
12 human 1936 EEF1D eukaryotic translation elon... XM_024447088.1 43.4% 43.4% 1_1095del;1731C>T
13 human 1936 EEF1D eukaryotic translation elon... NM_001130053.4 43.3% 43.4% 1_1098del;1734C>T
14 human 1936 EEF1D eukaryotic translation elon... NM_032378.6 43.3% 43.4% 1_1098del;1734C>T
15 human 1936 EEF1D eukaryotic translation elon... XM_005250820.4 43.3% 43.4% 1_1098del;1734C>T
16 human 1936 EEF1D eukaryotic translation elon... XM_005250823.1 43.3% 43.4% 1_1098del;1734C>T
17 human 1936 EEF1D eukaryotic translation elon... XM_006716522.1 43.3% 43.4% 1_1098del;1734C>T
18 human 1936 EEF1D eukaryotic translation elon... XM_006716523.2 43.3% 43.4% 1_1098del;1734C>T
19 human 1936 EEF1D eukaryotic translation elon... XM_006716524.1 43.3% 43.4% 1_1098del;1734C>T
20 human 1936 EEF1D eukaryotic translation elon... XM_011516905.2 43.3% 43.4% 1_1098del;1734C>T
21 human 1936 EEF1D eukaryotic translation elon... XM_011516906.3 43.3% 43.4% 1_1098del;1734C>T
22 human 1936 EEF1D eukaryotic translation elon... XM_017013170.2 43.3% 43.4% 1_1098del;1734C>T
23 human 1936 EEF1D eukaryotic translation elon... XM_006716520.4 39.6% 39.7% 1_1098del;1215_1216ins72;1662C>T
24 human 1936 EEF1D eukaryotic translation elon... XM_017013172.1 39.6% 39.7% 1_1098del;1215_1216ins72;1662C>T
25 human 1936 EEF1D eukaryotic translation elon... XM_024447089.1 39.6% 39.7% 1_1098del;1215_1216ins72;1662C>T
26 mouse 66656 Eef1d eukaryotic translation elon... NM_001285429.1 86.4% 92.5% (many diffs)
27 mouse 66656 Eef1d eukaryotic translation elon... NM_001285430.1 86.4% 92.5% (many diffs)
28 mouse 66656 Eef1d eukaryotic translation elon... NM_023240.3 86.4% 92.5% (many diffs)
29 mouse 66656 Eef1d eukaryotic translation elon... NM_001285434.1 81.2% 85.1% (many diffs)
30 mouse 66656 Eef1d eukaryotic translation elon... XM_006521268.3 81% 86.6% (many diffs)
31 mouse 66656 Eef1d eukaryotic translation elon... XM_006521273.2 81% 86.6% (many diffs)
32 mouse 66656 Eef1d eukaryotic translation elon... NM_001285431.1 78.5% 83.9% (many diffs)
33 mouse 66656 Eef1d eukaryotic translation elon... NM_001285432.1 78.5% 83.9% (many diffs)
34 mouse 66656 Eef1d eukaryotic translation elon... NM_001285433.1 78.5% 83.9% (many diffs)
35 mouse 66656 Eef1d eukaryotic translation elon... XM_006521272.2 76.9% 82.2% (many diffs)
36 mouse 66656 Eef1d eukaryotic translation elon... XM_006521271.1 76.6% 82% (many diffs)
37 mouse 66656 Eef1d eukaryotic translation elon... XM_006521269.2 72.5% 77.6% (many diffs)
38 mouse 66656 Eef1d eukaryotic translation elon... XM_017316725.1 72.3% 77.3% (many diffs)
39 mouse 66656 Eef1d eukaryotic translation elon... XM_006521275.2 69.8% 74.6% (many diffs)
40 mouse 66656 Eef1d eukaryotic translation elon... XM_006521274.1 69.6% 74.4% (many diffs)
41 mouse 66656 Eef1d eukaryotic translation elon... XM_006521264.3 36.9% 39.4% (many diffs)
42 mouse 66656 Eef1d eukaryotic translation elon... NM_029663.2 36.8% 39.3% (many diffs)
43 mouse 66656 Eef1d eukaryotic translation elon... XM_006521262.3 35.8% 38.3% (many diffs)
44 mouse 66656 Eef1d eukaryotic translation elon... XM_006521261.3 35.8% 38.2% (many diffs)
45 mouse 66656 Eef1d eukaryotic translation elon... XM_006521266.3 35.8% 38.2% (many diffs)
46 mouse 66656 Eef1d eukaryotic translation elon... XM_006521267.3 35.8% 38.2% (many diffs)
47 mouse 66656 Eef1d eukaryotic translation elon... XM_006521265.3 33.4% 35.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
909
ORF length:
843
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tacaaacttc ctagcacatg agaagatctg gttcgacaag ttcaaatatg
121acgacgcaga aaggagattc tacgagcaga tgaacgggcc tgtggcaggt gcctcccgcc
181aggagaacgg cgccagcgtg atcctccgtg acattgcgag agccagagag aacatccaga
241aatccctggc tggaagctca ggccccgggg cctccagcgg caccagcgga gaccacggtg
301agctcgtcgt ccggattgcc agtctggaag tggagaacca gagtctgcgt ggcgtggtac
361aggagctgca gcaggccatc tccaagctgg aggcccggct gaacgtgctg gagaagagct
421cgcctggcca ccgggccacg gccccacaga cccagcacgt atctcCCATG CGCCAAGTGG
481AGCCCCCAGC CAAGAAGCCA GCCACACCAG CAGAGGATGA CGAGGATGAT GACATTGACC
541TGTTTGGCAG TGACAATGAG GAGGAGGACA AGGAGGCGGC ACAGCTGCGG GAGGAGCGGC
601TACGGCAGTA CGCGGAGAAG AAGGCCAAGA AGCCTGCACT GGTGGCCAAG TCCTCCATCC
661TGCTGGATGT CAAGCCTTGG GATGATGAGA CGGACATGGC TCAGCTGGAG GCCTGTGTGC
721GCTCTATCCA GCTGGACGGG CTGGTCTGGG GGGCTTCCAA GCTGGTGCCC GTGGGCTACG
781GTATCCGGAA GCTACAGATT CAGTGTGTGG TGGAGGACGA CAAGGTGGGG ACAGACTTGC
841TGGAGGAGGA GATCACCAAG TTTGAGGAGC ACGTGCAGAG TGTCGATATC GCAGCTTTCA
901ACAAGATCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
961CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1021TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCGTC CTTTTTTCTT TTCATCTAAC
1081GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)