Construct: ORF TRCN0000470997
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011681.1_s317c1
- Derived from:
- ccsbBroadEn_15404
- DNA Barcode:
- ATCGTCCTTTTTTCTTTTCATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF1D (1936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470997
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130055.3 | 99.8% | 100% | 636C>T |
2 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130057.3 | 99.8% | 100% | 636C>T |
3 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001289950.3 | 99.8% | 100% | 636C>T |
4 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001960.6 | 99.8% | 100% | 636C>T |
5 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716525.1 | 99.8% | 100% | 636C>T |
6 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001195203.3 | 93.1% | 93.2% | 230_231ins57;579C>T |
7 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130056.4 | 91.3% | 91.4% | 117_118ins72;564C>T |
8 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001317743.3 | 91.3% | 91.4% | 117_118ins72;564C>T |
9 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001330646.2 | 91.3% | 91.4% | 117_118ins72;564C>T |
10 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716519.4 | 43.4% | 43.4% | 1_1095del;1731C>T |
11 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_017013171.1 | 43.4% | 43.4% | 1_1095del;1731C>T |
12 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_024447088.1 | 43.4% | 43.4% | 1_1095del;1731C>T |
13 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_001130053.4 | 43.3% | 43.4% | 1_1098del;1734C>T |
14 | human | 1936 | EEF1D | eukaryotic translation elon... | NM_032378.6 | 43.3% | 43.4% | 1_1098del;1734C>T |
15 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_005250820.4 | 43.3% | 43.4% | 1_1098del;1734C>T |
16 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_005250823.1 | 43.3% | 43.4% | 1_1098del;1734C>T |
17 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716522.1 | 43.3% | 43.4% | 1_1098del;1734C>T |
18 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716523.2 | 43.3% | 43.4% | 1_1098del;1734C>T |
19 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716524.1 | 43.3% | 43.4% | 1_1098del;1734C>T |
20 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_011516905.2 | 43.3% | 43.4% | 1_1098del;1734C>T |
21 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_011516906.3 | 43.3% | 43.4% | 1_1098del;1734C>T |
22 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_017013170.2 | 43.3% | 43.4% | 1_1098del;1734C>T |
23 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_006716520.4 | 39.6% | 39.7% | 1_1098del;1215_1216ins72;1662C>T |
24 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_017013172.1 | 39.6% | 39.7% | 1_1098del;1215_1216ins72;1662C>T |
25 | human | 1936 | EEF1D | eukaryotic translation elon... | XM_024447089.1 | 39.6% | 39.7% | 1_1098del;1215_1216ins72;1662C>T |
26 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_001285429.1 | 86.4% | 92.5% | (many diffs) |
27 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_001285430.1 | 86.4% | 92.5% | (many diffs) |
28 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_023240.3 | 86.4% | 92.5% | (many diffs) |
29 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_001285434.1 | 81.2% | 85.1% | (many diffs) |
30 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521268.3 | 81% | 86.6% | (many diffs) |
31 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521273.2 | 81% | 86.6% | (many diffs) |
32 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_001285431.1 | 78.5% | 83.9% | (many diffs) |
33 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_001285432.1 | 78.5% | 83.9% | (many diffs) |
34 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_001285433.1 | 78.5% | 83.9% | (many diffs) |
35 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521272.2 | 76.9% | 82.2% | (many diffs) |
36 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521271.1 | 76.6% | 82% | (many diffs) |
37 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521269.2 | 72.5% | 77.6% | (many diffs) |
38 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_017316725.1 | 72.3% | 77.3% | (many diffs) |
39 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521275.2 | 69.8% | 74.6% | (many diffs) |
40 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521274.1 | 69.6% | 74.4% | (many diffs) |
41 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521264.3 | 36.9% | 39.4% | (many diffs) |
42 | mouse | 66656 | Eef1d | eukaryotic translation elon... | NM_029663.2 | 36.8% | 39.3% | (many diffs) |
43 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521262.3 | 35.8% | 38.3% | (many diffs) |
44 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521261.3 | 35.8% | 38.2% | (many diffs) |
45 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521266.3 | 35.8% | 38.2% | (many diffs) |
46 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521267.3 | 35.8% | 38.2% | (many diffs) |
47 | mouse | 66656 | Eef1d | eukaryotic translation elon... | XM_006521265.3 | 33.4% | 35.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 909
- ORF length:
- 843
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tacaaacttc ctagcacatg agaagatctg gttcgacaag ttcaaatatg 121 acgacgcaga aaggagattc tacgagcaga tgaacgggcc tgtggcaggt gcctcccgcc 181 aggagaacgg cgccagcgtg atcctccgtg acattgcgag agccagagag aacatccaga 241 aatccctggc tggaagctca ggccccgggg cctccagcgg caccagcgga gaccacggtg 301 agctcgtcgt ccggattgcc agtctggaag tggagaacca gagtctgcgt ggcgtggtac 361 aggagctgca gcaggccatc tccaagctgg aggcccggct gaacgtgctg gagaagagct 421 cgcctggcca ccgggccacg gccccacaga cccagcacgt atctcCCATG CGCCAAGTGG 481 AGCCCCCAGC CAAGAAGCCA GCCACACCAG CAGAGGATGA CGAGGATGAT GACATTGACC 541 TGTTTGGCAG TGACAATGAG GAGGAGGACA AGGAGGCGGC ACAGCTGCGG GAGGAGCGGC 601 TACGGCAGTA CGCGGAGAAG AAGGCCAAGA AGCCTGCACT GGTGGCCAAG TCCTCCATCC 661 TGCTGGATGT CAAGCCTTGG GATGATGAGA CGGACATGGC TCAGCTGGAG GCCTGTGTGC 721 GCTCTATCCA GCTGGACGGG CTGGTCTGGG GGGCTTCCAA GCTGGTGCCC GTGGGCTACG 781 GTATCCGGAA GCTACAGATT CAGTGTGTGG TGGAGGACGA CAAGGTGGGG ACAGACTTGC 841 TGGAGGAGGA GATCACCAAG TTTGAGGAGC ACGTGCAGAG TGTCGATATC GCAGCTTTCA 901 ACAAGATCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 961 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1021 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCGTC CTTTTTTCTT TTCATCTAAC 1081 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt