Construct: ORF TRCN0000471024

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010579.1_s317c1
Derived from:
ccsbBroadEn_11287
DNA Barcode:
TACCCCATGATGCGGCAACCTTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NPFF (8620)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471024
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8620 NPFF neuropeptide FF-amide pepti... NM_001320296.2 100% 100%
2 human 8620 NPFF neuropeptide FF-amide pepti... NM_003717.4 75.3% 65.3% (many diffs)
3 human 114108587 ATF7-NPFF ATF7-NPFF readthrough NR_159377.1 14.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
441
ORF length:
348
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGTTCC GCAGCCTCCT ACCACTTGCC
121CCTGGAAGCC AGTCCCTTCC CCTTGTGACT TACGTGTCCA GGGTATTTGC CCATCTTCCT
181TCCCTGATAC CCCCTTGGCA CAGGAGGAAG ACAGCGAACC CCTCCCACCA CAGGATGCCC
241AGACCTCTGG GTCACTGTTG CACTACCTGC TCCAGGCAAT GGAGAGACCT GGCCGGAGCC
301AAGCCTTCCT GTTTCAGCCC CAGAGGTTTG GCAGAAATAC CCAGGGATCC TGGAGGAATG
361AATGGCTGAG TCCCCGGGCT GGAGAGGGGC TGAATTCCCA GTTCTGGAGC CTGGCTGCCC
421CTCAACGCTT TGGGAAGAAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
481CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
541AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACC CCATGATGCG
601GCAACCTTGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)