Construct: ORF TRCN0000471024
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010579.1_s317c1
- Derived from:
- ccsbBroadEn_11287
- DNA Barcode:
- TACCCCATGATGCGGCAACCTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPFF (8620)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471024
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8620 | NPFF | neuropeptide FF-amide pepti... | NM_001320296.2 | 100% | 100% | |
| 2 | human | 8620 | NPFF | neuropeptide FF-amide pepti... | NM_003717.4 | 75.3% | 65.3% | (many diffs) |
| 3 | human | 114108587 | ATF7-NPFF | ATF7-NPFF readthrough | NR_159377.1 | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 441
- ORF length:
- 348
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGTTCC GCAGCCTCCT ACCACTTGCC 121 CCTGGAAGCC AGTCCCTTCC CCTTGTGACT TACGTGTCCA GGGTATTTGC CCATCTTCCT 181 TCCCTGATAC CCCCTTGGCA CAGGAGGAAG ACAGCGAACC CCTCCCACCA CAGGATGCCC 241 AGACCTCTGG GTCACTGTTG CACTACCTGC TCCAGGCAAT GGAGAGACCT GGCCGGAGCC 301 AAGCCTTCCT GTTTCAGCCC CAGAGGTTTG GCAGAAATAC CCAGGGATCC TGGAGGAATG 361 AATGGCTGAG TCCCCGGGCT GGAGAGGGGC TGAATTCCCA GTTCTGGAGC CTGGCTGCCC 421 CTCAACGCTT TGGGAAGAAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACC CCATGATGCG 601 GCAACCTTGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt