Construct: ORF TRCN0000471038
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006123.1_s317c1
- Derived from:
- ccsbBroadEn_07945
- DNA Barcode:
- CGAAAGGACCTTGTTAACAGTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCA (25801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471038
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25801 | GCA | grancalcin | NM_012198.5 | 99.8% | 100% | 486T>A |
| 2 | human | 25801 | GCA | grancalcin | NM_001330265.1 | 91.9% | 89.6% | (many diffs) |
| 3 | human | 25801 | GCA | grancalcin | NM_001330266.1 | 91.9% | 89.6% | (many diffs) |
| 4 | human | 25801 | GCA | grancalcin | NM_001330267.1 | 91.9% | 89.6% | (many diffs) |
| 5 | human | 25801 | GCA | grancalcin | NM_001330270.2 | 91% | 91.2% | 0_1ins57;429T>A |
| 6 | human | 25801 | GCA | grancalcin | NM_001330271.2 | 91% | 91.2% | 0_1ins57;429T>A |
| 7 | human | 25801 | GCA | grancalcin | XM_005246446.3 | 90.7% | 89.7% | (many diffs) |
| 8 | human | 25801 | GCA | grancalcin | NM_001330268.1 | 87.9% | 87.2% | (many diffs) |
| 9 | human | 25801 | GCA | grancalcin | XM_006712400.4 | 84% | 80.8% | (many diffs) |
| 10 | human | 25801 | GCA | grancalcin | XM_011510926.2 | 82.6% | 81.7% | (many diffs) |
| 11 | human | 25801 | GCA | grancalcin | XM_017003767.1 | 82.6% | 81.7% | (many diffs) |
| 12 | human | 25801 | GCA | grancalcin | XM_006712398.4 | 80.5% | 78.9% | (many diffs) |
| 13 | human | 25801 | GCA | grancalcin | XM_011510928.2 | 49.9% | 48.9% | (many diffs) |
| 14 | human | 25801 | GCA | grancalcin | XM_017003769.2 | 42.1% | 40.7% | (many diffs) |
| 15 | mouse | 227960 | Gca | grancalcin | NM_145523.3 | 82.6% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctacccggga tacggaggag ggtttggaaa ttttagcatt caggtgccag 121 gaatgcagat gggacagcca gtgccagaaa caggcccagc tatactcctc gatggatact 181 ctgggccagc atattcagac acttattccT CAGCTGGTGA CTCCGTGTAT ACTTACTTCA 241 GTGCTGTTGC TGGACAGGAT GGTGAAGTGG ATGCTGAAGA ACTTCAGAGA TGTTTGACAC 301 AGTCTGGAAT TAATGGAACT TACTCTCCCT TCAGTTTGGA AACCTGCAGA ATTATGATTG 361 CCATGTTGGA TAGAGATCAC ACAGGAAAAA TGGGATTTAA TGCATTCAAA GAGCTATGGG 421 CAGCTCTTAA TGCCTGGAAG GAAAACTTCA TGACTGTTGA TCAAGATGGA AGTGGCACAG 481 TAGAACATCA TGAGTTGCGT CAAGCCATTG GTCTTATGGG TTATAGGTTG AGTCCTCAAA 541 CATTAACTAC AATTGTTAAA CGTTATAGCA AGAATGGCAG AATTTTCTTT GATGATTATG 601 TTGCTTGCTG TGTGAAGCTT CGAGCATTGA CAGATTTCTT TAGGAAAAGA GACCACTTGC 661 AACAAGGGTC TGCGAATTTC ATATATGACG ATTTTTTGCA GGGCACTATG GCAATTTGCC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GACGAAAGGA CCTTGTTAAC AGTTTAACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt