Construct: ORF TRCN0000471053

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009960.1_s317c1
Derived from:
ccsbBroadEn_10964
DNA Barcode:
CCTGGATTATATCCTACGGAACCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MEN1 (4221)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471053
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4221 MEN1 menin 1 NM_001370262.1 99.9% 100% 1149C>T
2 human 4221 MEN1 menin 1 NM_001370263.1 99.9% 100% 1149C>T
3 human 4221 MEN1 menin 1 NM_001370259.1 94.2% 94.2% 548_652del;1254C>T
4 human 4221 MEN1 menin 1 NM_001370260.1 94.2% 94.2% 548_652del;1254C>T
5 human 4221 MEN1 menin 1 NM_001370261.1 94.2% 94.2% 548_652del;1254C>T
6 human 4221 MEN1 menin 1 NM_130799.2 94.2% 94.2% 548_652del;1254C>T
7 human 4221 MEN1 menin 1 NM_000244.3 93.4% 93.4% 444_458del;563_667del;1269C>T
8 human 4221 MEN1 menin 1 NM_130800.2 93.4% 93.4% 444_458del;563_667del;1269C>T
9 human 4221 MEN1 menin 1 NM_130801.2 93.4% 93.4% 444_458del;563_667del;1269C>T
10 human 4221 MEN1 menin 1 NM_130802.2 93.4% 93.4% 444_458del;563_667del;1269C>T
11 human 4221 MEN1 menin 1 NM_130803.2 93.4% 93.4% 444_458del;563_667del;1269C>T
12 human 4221 MEN1 menin 1 NM_130804.2 93.4% 93.4% 444_458del;563_667del;1269C>T
13 human 4221 MEN1 menin 1 NM_001370251.1 88.1% 88% 548_652del;1049_1174del;1380C>T
14 human 4221 MEN1 menin 1 XM_011545040.1 88% 87.8% (many diffs)
15 human 4221 MEN1 menin 1 XM_011545041.2 88% 87.8% (many diffs)
16 human 4221 MEN1 menin 1 XM_017017765.1 87.3% 87.2% (many diffs)
17 human 4221 MEN1 menin 1 XM_017017766.1 87.3% 87.2% (many diffs)
18 human 4221 MEN1 menin 1 XM_017017767.2 87.3% 87.2% (many diffs)
19 human 4221 MEN1 menin 1 XM_017017768.1 87.3% 87.2% (many diffs)
20 mouse 17283 Men1 multiple endocrine neoplasia 1 NM_001168489.1 83.5% 90.8% (many diffs)
21 mouse 17283 Men1 multiple endocrine neoplasia 1 NM_008583.2 83.5% 90.8% (many diffs)
22 mouse 17283 Men1 multiple endocrine neoplasia 1 NM_001168488.1 82.7% 89.9% (many diffs)
23 mouse 17283 Men1 multiple endocrine neoplasia 1 NM_001168490.1 75.6% 81.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1791
ORF length:
1725
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gctgaaggcc gcccagaaga cgctgttccc gctgcgctcc atcgacgacg
121tggtgcgcct gtttgctgcc gagctgggcc gagaggagcc ggacctggtg ctcctttcct
181tggtgctggg cttcgtggag cattttctgg ctgtcaaccg cgtcatccct accaacgttc
241ccgagctcac cttccagccc agccccgccc ccgacccgcc tggcggcctc acctactttc
301ccgtggccga cctgtctatc atcgccgccc tctatgcccg cttcaccgcc cagatccgag
361gcgccgtcga cctgtccctc tatcctcgag aagggggtgt ctccagccgt gagctggtga
421agaaggtctc cgatgtcata tggaacagcc tcagccgctc ctacttcaag gatcgggccc
481acatccagtc cctcttcagc ttcatcacag gcaccaaatt ggacagctcc ggtgtggcct
541ttgctgtggt tggggcctgc caggccctgg gtctccggga tgtccacctc gccctgtctg
601aggatcatgc ctggagctgg ctgtacctga aaggatcata catgcgctgt gaccgcaaga
661tggaggtggc gttcatggtg tgtgccatca acccttccat tgacctgcac accgactcgc
721tggagcttct gcagctgcag cagaagctgc tctggctgct ctatgacctg ggacatctgg
781aaaggtaccc catggcctta gggaacctgg cagatctaga ggagctggag cccacccctg
841gccggccaga cccactcacc ctctaccaca agggcattgc ctcagccaag acctactatc
901gggatgaaca catctacccc tacatgtacc tggctggcta ccactgtcgc aaccgcaatg
961tgcgggaagc cctgcaggcc tgggcggaca cggccactgt catccaggac tacaactact
1021gccgggaaga cgaggagatc tacaaggagt tctttgaagt agccaatgat gtcatcccca
1081acctgctgaa ggaggcagcc agcttgctgg aggcgggcga ggagcggccg ggggagcaaa
1141gccagggcac ccagagccaa ggttccgccc tccaggaccc tgagtgcttc gcccacctgc
1201tgcgattcta cgatggcatc tgcaaatggg aggagggcag tcccacgcct gtgctgcacg
1261tgggctgggc cacctttctt gtgcagtccc taggccgttt tgagggacag gtgcggcaga
1321aggtgcgcat agtgagccga gaggccgagg cggccgaggc cgaggagccg tggggcgagg
1381aagcccggga aggccggcgg cggggcccac ggcgggagtc CAAGCCAGAG GAGCCCCCGC
1441CGCCCAAGAA GCCAGCACTG GACAAGGGCC TGGGCACCGG CCAGGGTGCA GTGTCAGGAC
1501CCCCCCGGAA GCCTCCTGGG ACTGTCGCTG GCACAGCCCG AGGCCCTGAA GGTGGCAGCA
1561CGGCTCAGGT GCCAGCACCC GCAGCATCAC CACCGCCGGA GGGTCCAGTG CTCACTTTCC
1621AGAGTGAGAA GATGAAGGGC ATGAAGGAGC TGCTGGTGGC CACCAAGATC AACTCGAGCG
1681CCATCAAGCT GCAACTCACG GCACAGTCGC AAGTGCAGAT GAAGAAGCAG AAAGTGTCCA
1741CCCCTAGTGA CTACACTCTG TCTTTCCTCA AGCGGCAGCG CAAAGGCCTC TGCCCAACTT
1801TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1861CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1921CTTGTGGAAA GGACGACCTG GATTATATCC TACGGAACCA ACGCGTTAAG TCgacaatca
1981acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)