Construct: ORF TRCN0000471053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009960.1_s317c1
- Derived from:
- ccsbBroadEn_10964
- DNA Barcode:
- CCTGGATTATATCCTACGGAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEN1 (4221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471053
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4221 | MEN1 | menin 1 | NM_001370262.1 | 99.9% | 100% | 1149C>T |
| 2 | human | 4221 | MEN1 | menin 1 | NM_001370263.1 | 99.9% | 100% | 1149C>T |
| 3 | human | 4221 | MEN1 | menin 1 | NM_001370259.1 | 94.2% | 94.2% | 548_652del;1254C>T |
| 4 | human | 4221 | MEN1 | menin 1 | NM_001370260.1 | 94.2% | 94.2% | 548_652del;1254C>T |
| 5 | human | 4221 | MEN1 | menin 1 | NM_001370261.1 | 94.2% | 94.2% | 548_652del;1254C>T |
| 6 | human | 4221 | MEN1 | menin 1 | NM_130799.2 | 94.2% | 94.2% | 548_652del;1254C>T |
| 7 | human | 4221 | MEN1 | menin 1 | NM_000244.3 | 93.4% | 93.4% | 444_458del;563_667del;1269C>T |
| 8 | human | 4221 | MEN1 | menin 1 | NM_130800.2 | 93.4% | 93.4% | 444_458del;563_667del;1269C>T |
| 9 | human | 4221 | MEN1 | menin 1 | NM_130801.2 | 93.4% | 93.4% | 444_458del;563_667del;1269C>T |
| 10 | human | 4221 | MEN1 | menin 1 | NM_130802.2 | 93.4% | 93.4% | 444_458del;563_667del;1269C>T |
| 11 | human | 4221 | MEN1 | menin 1 | NM_130803.2 | 93.4% | 93.4% | 444_458del;563_667del;1269C>T |
| 12 | human | 4221 | MEN1 | menin 1 | NM_130804.2 | 93.4% | 93.4% | 444_458del;563_667del;1269C>T |
| 13 | human | 4221 | MEN1 | menin 1 | NM_001370251.1 | 88.1% | 88% | 548_652del;1049_1174del;1380C>T |
| 14 | human | 4221 | MEN1 | menin 1 | XM_011545040.1 | 88% | 87.8% | (many diffs) |
| 15 | human | 4221 | MEN1 | menin 1 | XM_011545041.2 | 88% | 87.8% | (many diffs) |
| 16 | human | 4221 | MEN1 | menin 1 | XM_017017765.1 | 87.3% | 87.2% | (many diffs) |
| 17 | human | 4221 | MEN1 | menin 1 | XM_017017766.1 | 87.3% | 87.2% | (many diffs) |
| 18 | human | 4221 | MEN1 | menin 1 | XM_017017767.2 | 87.3% | 87.2% | (many diffs) |
| 19 | human | 4221 | MEN1 | menin 1 | XM_017017768.1 | 87.3% | 87.2% | (many diffs) |
| 20 | mouse | 17283 | Men1 | multiple endocrine neoplasia 1 | NM_001168489.1 | 83.5% | 90.8% | (many diffs) |
| 21 | mouse | 17283 | Men1 | multiple endocrine neoplasia 1 | NM_008583.2 | 83.5% | 90.8% | (many diffs) |
| 22 | mouse | 17283 | Men1 | multiple endocrine neoplasia 1 | NM_001168488.1 | 82.7% | 89.9% | (many diffs) |
| 23 | mouse | 17283 | Men1 | multiple endocrine neoplasia 1 | NM_001168490.1 | 75.6% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1791
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgaaggcc gcccagaaga cgctgttccc gctgcgctcc atcgacgacg 121 tggtgcgcct gtttgctgcc gagctgggcc gagaggagcc ggacctggtg ctcctttcct 181 tggtgctggg cttcgtggag cattttctgg ctgtcaaccg cgtcatccct accaacgttc 241 ccgagctcac cttccagccc agccccgccc ccgacccgcc tggcggcctc acctactttc 301 ccgtggccga cctgtctatc atcgccgccc tctatgcccg cttcaccgcc cagatccgag 361 gcgccgtcga cctgtccctc tatcctcgag aagggggtgt ctccagccgt gagctggtga 421 agaaggtctc cgatgtcata tggaacagcc tcagccgctc ctacttcaag gatcgggccc 481 acatccagtc cctcttcagc ttcatcacag gcaccaaatt ggacagctcc ggtgtggcct 541 ttgctgtggt tggggcctgc caggccctgg gtctccggga tgtccacctc gccctgtctg 601 aggatcatgc ctggagctgg ctgtacctga aaggatcata catgcgctgt gaccgcaaga 661 tggaggtggc gttcatggtg tgtgccatca acccttccat tgacctgcac accgactcgc 721 tggagcttct gcagctgcag cagaagctgc tctggctgct ctatgacctg ggacatctgg 781 aaaggtaccc catggcctta gggaacctgg cagatctaga ggagctggag cccacccctg 841 gccggccaga cccactcacc ctctaccaca agggcattgc ctcagccaag acctactatc 901 gggatgaaca catctacccc tacatgtacc tggctggcta ccactgtcgc aaccgcaatg 961 tgcgggaagc cctgcaggcc tgggcggaca cggccactgt catccaggac tacaactact 1021 gccgggaaga cgaggagatc tacaaggagt tctttgaagt agccaatgat gtcatcccca 1081 acctgctgaa ggaggcagcc agcttgctgg aggcgggcga ggagcggccg ggggagcaaa 1141 gccagggcac ccagagccaa ggttccgccc tccaggaccc tgagtgcttc gcccacctgc 1201 tgcgattcta cgatggcatc tgcaaatggg aggagggcag tcccacgcct gtgctgcacg 1261 tgggctgggc cacctttctt gtgcagtccc taggccgttt tgagggacag gtgcggcaga 1321 aggtgcgcat agtgagccga gaggccgagg cggccgaggc cgaggagccg tggggcgagg 1381 aagcccggga aggccggcgg cggggcccac ggcgggagtc CAAGCCAGAG GAGCCCCCGC 1441 CGCCCAAGAA GCCAGCACTG GACAAGGGCC TGGGCACCGG CCAGGGTGCA GTGTCAGGAC 1501 CCCCCCGGAA GCCTCCTGGG ACTGTCGCTG GCACAGCCCG AGGCCCTGAA GGTGGCAGCA 1561 CGGCTCAGGT GCCAGCACCC GCAGCATCAC CACCGCCGGA GGGTCCAGTG CTCACTTTCC 1621 AGAGTGAGAA GATGAAGGGC ATGAAGGAGC TGCTGGTGGC CACCAAGATC AACTCGAGCG 1681 CCATCAAGCT GCAACTCACG GCACAGTCGC AAGTGCAGAT GAAGAAGCAG AAAGTGTCCA 1741 CCCCTAGTGA CTACACTCTG TCTTTCCTCA AGCGGCAGCG CAAAGGCCTC TGCCCAACTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGACCTG GATTATATCC TACGGAACCA ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt