Construct: ORF TRCN0000471070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001499.1_s317c1
- Derived from:
- ccsbBroadEn_07273
- DNA Barcode:
- CTCGTCCCCAAGACGGGTGCAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASMTL (8623)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471070
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | NM_004192.4 | 99.6% | 99.8% | (many diffs) |
| 2 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | XM_005274434.3 | 99.6% | 99.8% | (many diffs) |
| 3 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | XM_005274783.3 | 99.6% | 99.8% | (many diffs) |
| 4 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | NM_001173474.2 | 97.1% | 97.2% | (many diffs) |
| 5 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | NM_001173473.1 | 90.3% | 90.4% | (many diffs) |
| 6 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | XM_011545638.2 | 85.3% | 85.1% | (many diffs) |
| 7 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | XM_011546185.2 | 85.3% | 85.1% | (many diffs) |
| 8 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | XM_017029919.2 | 82.1% | 82.2% | (many diffs) |
| 9 | human | 8623 | ASMTL | acetylserotonin O-methyltra... | XM_017030084.2 | 82.1% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1929
- ORF length:
- 1863
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gctgtgcccg gtgattggga agctgctgca caagcgcgtg gtgctggcca 121 gcgcctcccc acgccgtcag gagatcctca gcaacgcggg tctcaggttt gaggtggtcc 181 cctccaagtt taaagagaag ctggacaaag cctccttcgc tactccgtat gggtacgcca 241 tggagaccgc caagcagaag gccctggagg tggccaaccg gctgtaccag aaagacctgc 301 gggcccccga cgtggtcatt ggagcggaca cgatcgtgac ggtcgggggg ctgattctgg 361 agaagccggt ggacaagcag gacgcctaca ggatgctgtc ccggttgagt gggagagaac 421 acagcgtgtt cacaggtgtc gcgatcgtcc actgctccag caaagaccat cagctggaca 481 ccagggtctc ggaattctac gaggaaacga aggtgaagtt ctcggagctg tccgaggagc 541 tgctctggga atacgtccac agcggggagc ccatggacaa agctggcggc tacgggatcc 601 aggccctggg cggcatgctg gtggagtccg tacacgggga ctttctgaac gtggtgggat 661 tcccgctgaa ccacttctgc aagcagctgg tgaagctcta ctacccgccc cgcccggagg 721 acctgcggcg gagtgtcaag cacgactcca tcccggccgc ggacaccttc gaagacctca 781 gtgacgtgga ggggggtggc tcggagccca ctcagaggga cgcgggcagc cgcgatgaga 841 aagccgaggc gggagaggcg ggacaggcca cggcagaggc tgagtgtcac aggactcggg 901 agaccctgcc tccgttcccg acacgcctcc tggagctgat tgagggcttt atgctatcca 961 agggcctgct caccgcttgc aaactgaagg tgttcgattt gttaaaagat gaagcacccc 1021 agaaggctgc ggatattgcc agcaaagtgg acgcctctgc gtgtggaatg gagaggcttc 1081 tggacatctg tgctgccatg gggctcctgg agaagacaga gcaaggttac agtaacacag 1141 agacagcgaa cgtctacctg gcatcggatg gcgaatactc tctgcacggc ttcatcatgc 1201 acaataatga cctcacatgg aacctcttta catacctgga gtttgccatc cgagagggaa 1261 caaaccagca ccacagggcg ttggggaaga aggcggaaga tctgttccag gatgcgtact 1321 accagagccc ggagacgcgg ctgaggttca tgcgggccat gcacggcatg acgaagctga 1381 ctgcgtgcca ggtggccacg gccttcaatc tgtcccgctt ctcctccgcc tgcgacgtgg 1441 gaggctgcac cggtgcactg gcccgagagc tggcccgtga gtacccTCGT ATGCAGGTGA 1501 CTGTGTTTGA CCTCCCAGAC ATTATCGAGC TGGCCGCCCA CTTCCAACCC CCCGGACCGC 1561 AGGCAGTGCA GATCCACTTC GCAGCAGGTG ACTTTTTCAG GGACCCCCTC CCCAGCGCTG 1621 AGCTGTACGT CCTGTGCCGG ATCCTGCATG ACTGGCCAGA CGACAAAGTC CACAAGTTAC 1681 TCAGCAAGGT CGCCGAGAGC TGCAAGCCAG GGGCCGGCCT GCTGCTGGTG GAGACGCTCC 1741 TGGATGAGGA GAAGAGGGTG GCGCAGCGCG CCCTGATGCA GTCACTGAAC ATGCTGGTGC 1801 AGACTGAAGG CAAGGAGCGG AGCCTGGGCG AGTATCAGTG CTTGCTGGAG CTGCACGGCT 1861 TCCACCAGGT GCAGGTGGTG CACTTGGGGG GTGTCCTGGA TGCCATCTTG GCCACCAAAG 1921 TGGCCCCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1981 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2041 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCGTC CCCAAGACGG GTGCAAATAC 2101 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt