Construct: ORF TRCN0000471076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013254.1_s317c1
- Derived from:
- ccsbBroadEn_09875
- DNA Barcode:
- ATTTAGCTGAGTGTCTCGTTGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF182 (221687)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471076
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221687 | RNF182 | ring finger protein 182 | NM_001165032.1 | 100% | 100% | |
| 2 | human | 221687 | RNF182 | ring finger protein 182 | NM_001165033.1 | 100% | 100% | |
| 3 | human | 221687 | RNF182 | ring finger protein 182 | NM_001165034.1 | 100% | 100% | |
| 4 | human | 221687 | RNF182 | ring finger protein 182 | NM_152737.4 | 100% | 100% | |
| 5 | human | 221687 | RNF182 | ring finger protein 182 | XM_017010450.2 | 100% | 100% | |
| 6 | mouse | 328234 | Rnf182 | ring finger protein 182 | NM_183204.4 | 88.5% | 98.3% | (many diffs) |
| 7 | mouse | 328234 | Rnf182 | ring finger protein 182 | XM_006516933.3 | 88.5% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagtcaacct cctgaagaca ctgcggagtc tcaggcctct gatgagctgg 121 agtgcaaaat ctgttacaat cgatacaatc tgaaacagag gaaacccaaa gtgctggagt 181 gttgtcatag ggtttgtgcc aaatgcctct acaagatcat agactttggg gactccccac 241 aaggtgtcat tgtctgtcct ttctgcaggt ttgagacgtg cctGCCAGAT GATGAAGTTA 301 GTAGCCTGCC CGATGACAAC AACATCCTTG TAAACTTGAC TTGTGGAGGC AAAGGGAAGA 361 AGTGCCTGCC AGAGAACCCT ACTGAGCTGC TGCTCACCCC CAAGAGGCTG GCCTCTCTGG 421 TCAGTCCTTC TCACACGTCC TCCAACTGCC TGGTCATAAC CATCATGGAG GTGCAGAGAG 481 AGAGCTCCCC GTCCCTGAGC TCCACTCCTG TGGTAGAATT TTATAGGCCT GCGAGTTTCG 541 ACTCTGTCAC CACTGTGTCA CACAACTGGA CTGTGTGGAA CTGCACGTCC CTGCTGTTTC 601 AGACATCCAT CCGGGTGTTA GTGTGGTTGC TAGGTTTGCT CTACTTCAGC TCCTTACCCT 661 TAGGAATCTA CTTACTGGTG TCTAAGAAAG TCACCCTTGG GGTCGTCTTT GTCAGCCTGG 721 TCCCTTCGAG CCTCGTTATT CTTATGGTGT ATGGTTTTTG CCAGTGTGTT TGTCATGAAT 781 TTCTAGACTG TATGGCACCT CCTTCTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATTTAGCT 961 GAGTGTCTCG TTGAACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt