Construct: ORF TRCN0000471077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004386.1_s317c1
- Derived from:
- ccsbBroadEn_03882
- DNA Barcode:
- GCAGGATCGCGCATTTGTCGATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM3A (60343)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471077
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001171132.3 | 100% | 100% | |
| 2 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001282312.2 | 100% | 100% | |
| 3 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_021806.4 | 100% | 100% | |
| 4 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001363822.2 | 97% | 97% | 334_354del |
| 5 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_006724832.3 | 96.6% | 96.6% | 334_357del |
| 6 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001171134.2 | 92.6% | 92.6% | 333_334ins51 |
| 7 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_005277879.4 | 92.6% | 92.6% | 333_334ins51 |
| 8 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001171133.3 | 82.8% | 81.3% | (many diffs) |
| 9 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_017029700.2 | 81.3% | 77.3% | (many diffs) |
| 10 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_017029701.1 | 81.3% | 77.3% | (many diffs) |
| 11 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_017029702.1 | 81.3% | 77.3% | (many diffs) |
| 12 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452419.1 | 81.3% | 77.3% | (many diffs) |
| 13 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452420.1 | 81.3% | 77.3% | (many diffs) |
| 14 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | NM_001282311.2 | 80.4% | 80.4% | 0_1ins56;72_169del |
| 15 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_005274716.3 | 80.4% | 80.4% | 0_1ins56;72_169del |
| 16 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452416.1 | 78.3% | 78.1% | 0_1ins56;72_169del;376_396del |
| 17 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452415.1 | 78% | 77.8% | 0_1ins56;72_169del;376_399del |
| 18 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_006724833.2 | 73.9% | 73.4% | 0_1ins56;72_169del;375_376ins51 |
| 19 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XM_024452418.1 | 73.9% | 73.4% | 0_1ins56;72_169del;375_376ins51 |
| 20 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958803.1 | 35.1% | (many diffs) | |
| 21 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958800.1 | 34.4% | 1_344del;728_1047del;1355_2001del | |
| 22 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958801.1 | 32.5% | (many diffs) | |
| 23 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958799.1 | 32% | (many diffs) | |
| 24 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958798.1 | 31.2% | (many diffs) | |
| 25 | human | 60343 | FAM3A | FAM3 metabolism regulating ... | XR_002958802.1 | 30.1% | (many diffs) | |
| 26 | mouse | 66294 | Fam3a | family with sequence simila... | NM_001313725.1 | 85.8% | 90.8% | (many diffs) |
| 27 | mouse | 66294 | Fam3a | family with sequence simila... | NM_025473.4 | 85.8% | 90.8% | (many diffs) |
| 28 | mouse | 66294 | Fam3a | family with sequence simila... | XM_011247659.2 | 77.4% | 81.9% | (many diffs) |
| 29 | mouse | 66294 | Fam3a | family with sequence simila... | XM_011247660.2 | 64.1% | 66.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gttggcaggc cctctccgca ttgtggtcct agtcgtcagt gtgggtgtca 121 catggatcgt ggtcagcatc ctcctgggtg ggcctggcag tggctttcct cgcatccagc 181 aactcttcac cagtccagag agctcggtga ctgcagcgcc acgggccagg aagtacaagt 241 gtggcctgcc ccagccgtgt cctgaggagc acctggcctt ccgcgtggtc agcggggccg 301 ccaacgtcat tgGGCCCAAG ATCTGCCTCG AGGACAAGAT GCTGATGAGC AGCGTCAAGG 361 ACAACGTGGG CCGCGGGCTG AACATCGCCC TGGTGAACGG GGTCAGCGGC GAGCTCATCG 421 AGGCCCGGGC CTTTGACATG TGGGCCGGAG ATGTCAACGA CCTGTTGAAG TTTATTCGGC 481 CACTGCACGA AGGCACCCTG GTGTTCGTGG CATCCTACGA CGACCCAGCC ACCAAGATGA 541 ATGAAGAGAC CAGAAAGCTC TTCAGTGAGC TGGGCAGCAG GAACGCCAAG GAGCTGGCCT 601 TCCGGGACAG CTGGGTGTTT GTCGGGGCCA AGGGTGTGCA GAACAAGAGC CCCTTTGAGC 661 AGCACGTGAA GAACAGTAAG CACAGCAACA AGTACGAAGG CTGGCCCGAG GCGCTGGAGA 721 TGGAAGGCTG TATCCCGCGG AGAAGCACGG CCAGCTACCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 AGCAGGATCG CGCATTTGTC GATGCACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt