Construct: ORF TRCN0000471087
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004936.1_s317c1
- Derived from:
- ccsbBroadEn_02466
- DNA Barcode:
- GTATTTTATGACAACCATGGTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARPC1A (10552)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471087
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10552 | ARPC1A | actin related protein 2/3 c... | NM_006409.4 | 100% | 100% | |
| 2 | human | 10552 | ARPC1A | actin related protein 2/3 c... | NM_001190996.1 | 96.2% | 94% | 0_1ins40;24_25insAG |
| 3 | mouse | 56443 | Arpc1a | actin related protein 2/3 c... | NM_019767.2 | 90.8% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc actgcatcag tttttactag agccaatcac ctgtcatgcc tggaacaggg 121 atcgtactca gattgccctc agtcccaata atcacgaagt gcacatctat aagaagaacg 181 ggagccagtg ggtgaaagct catgaactca aggagcacaa cggacacatc acaggtattg 241 actgggctcc caagagcgac cgcattgtca cttgtggggc agaccgcaat gcctatgtct 301 ggagtcagaa agatggtgtt tggaagccaa ccctggtgat cctgagaatt aatcgcgcag 361 ctacttttgt gaagtggtcc cccctagaga acaaatttgc tgtgggaagt ggagcacgac 421 tcatttctgt ttgttacttt gagtctgaaa atgactggtg ggtgagcaag cacattaaaa 481 agccgattcg ctccacagtc ctcagcttgg attggcatcc caacaacgtt ttgctggcag 541 caggatcatg tgacttcaaa tgcagagtgt tttctgccta cattaaagaa gtggatgaaa 601 agccagccag cacgccctgg ggcagcaaga tgccttttgg gcagctgatg tcagagtttg 661 gtggcagtgg cactggtggc tgggtccacg gggtaagctt ctctgccagt gggagccgcc 721 tggcctgggt cagcCACGAC AGCACCGTGT CTGTTGCTGA TGCCTCAAAA AGTGTGCAGG 781 TCTCGACTCT GAAGACAGAG TTCCTGCCGC TCCTAAGTGT GTCATTTGTC TCAGAGAACA 841 GCGTCGTGGC TGCTGGCCAT GACTGCTGCC CAATGCTCTT TAACTACGAT GACCGCGGCT 901 GCCTGACCTT CGTCTCCAAG TTAGATATTC CAAAACAGAG CATCCAACGC AACATGTCTG 961 CCATGGAACG CTTCCGCAAC ATGGACAAGA GAGCCACAAC TGAGGACCGC AACACGGCCT 1021 TGGAGACGCT GCACCAGAAT AGCATCACTC AAGTCTCTAT TTATGAGGTG GACAAGCAAG 1081 ATTGTCGCAA ATTTTGCACT ACTGGCATCG ATGGAGCCAT GACAATTTGG GATTTCAAGA 1141 CCCTCGAGTC TTCCATCCAG GGCCTCCGGA TAATGTGCCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AGTATTTTAT GACAACCATG GTGTAACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt