Construct: ORF TRCN0000471091
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014468.1_s317c1
- Derived from:
- ccsbBroadEn_10918
- DNA Barcode:
- TGGACAAATGACTGTCGTACCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IHH (3549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471091
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3549 | IHH | Indian hedgehog signaling m... | NM_002181.4 | 71.1% | 71.2% | 1_354del;600G>A;1128T>C |
| 2 | mouse | 16147 | Ihh | Indian hedgehog | NM_010544.3 | 62.9% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 945
- ORF length:
- 879
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccagtggccc ggtgtgaagc tgcgggtgac cgagggctgg gacgaggacg 121 gccaccactc agaggagtcc ctgcattatg agggccgcgc ggtggacatc accacatcag 181 accgcgaccg caataagtat ggactgctgg cgcgcttggc agtggaggcc ggctttgact 241 gggtgtatta cgagtcaaag gcccacgtgc attgctccgt caagtccgag cactcggccg 301 cagccaagac aggcggctgc ttccctgccg gagcccaggt acgcctggag agtggggcgc 361 gtgtggcctt gtcagccgtg aggccgggag accgtgtgct ggccatgggg gaggatggga 421 gccccacctt cagcgatgtg ctcattttcc tggaccgcga gcctcacagg ctgagagcct 481 tccaggtcat cgagacTCAG GACCCCCCAC GCCGCCTGGC ACTCACACCC GCTCACCTGC 541 TCTTTACGGC TGACAATCAC ACGGAGCCGG CAGCCCGCTT CCGGGCCACA TTTGCCAGCC 601 ACGTGCAGCC TGGCCAGTAC GTGCTGGTGG CTGGGGTGCC AGGCCTGCAG CCTGCCCGCG 661 TGGCAGCTGT CTCTACACAC GTGGCCCTCG GGGCCTACGC CCCGCTCACA AAGCATGGGA 721 CACTGGTGGT GGAGGATGTG GTGGCATCCT GCTTCGCGGC CGTGGCTGAC CACCACCTGG 781 CTCAGTTGGC CTTCTGGCCC CTGAGACTCT TTCACAGCTT GGCATGGGGC AGCTGGACCC 841 CGGGGGAGGG TGTGCATTGG TACCCCCAGC TGCTCTACCG CCTGGGGCGT CTCCTGCTAG 901 AAGAGGGCAG CTTCCACCCA CTGGGCATGT CCGGGGCAGG GAGCTGCCCA ACTTTCTTGT 961 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1021 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1081 GAAAGGACGA TGGACAAATG ACTGTCGTAC CGTTACGCGT TAAGTCgaca atcaacctct 1141 ggattacaaa atttgtgaaa gatt