Construct: ORF TRCN0000471126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011690.1_s317c1
- Derived from:
- ccsbBroadEn_03758
- DNA Barcode:
- AGGGGACACCACATGGATCACCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP22 (56940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471126
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_020185.4 | 99.6% | 100% | 114C>T;516G>A |
| 2 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_001286555.1 | 85.7% | 83.9% | (many diffs) |
| 3 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_017011062.1 | 66.6% | 65.7% | (many diffs) |
| 4 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_011514757.2 | 56.5% | 53.3% | (many diffs) |
| 5 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104473.1 | 13.7% | (many diffs) | |
| 6 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104475.1 | 13.6% | (many diffs) | |
| 7 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104474.1 | 12.8% | (many diffs) | |
| 8 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | NM_134068.3 | 88.9% | 93.4% | (many diffs) |
| 9 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | NM_001037955.4 | 76.3% | 76.6% | (many diffs) |
| 10 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | XM_006516519.1 | 74.2% | 74.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaatgggatg aacaagatcc tgcccggcct gtacatcggc aacttcaaag 121 atgccagaga cgcggaacaa ttgagcaaga acaaggtgac acatattctg tctgtccatg 181 atagtgccag gcctatgttG GAGGGAGTTA AATACCTGTG CATCCCAGCA GCGGATTCAC 241 CATCTCAAAA CCTGACAAGA CATTTCAAAG AAAGTATTAA ATTCATTCAC GAGTGCCGGC 301 TCCGCGGTGA GAGCTGCCTT GTACACTGCC TGGCCGGGGT CTCCAGGAGC GTGACACTGG 361 TGATCGCATA CATCATGACC GTCACTGACT TTGGCTGGGA GGATGCCCTG CACACCGTGC 421 GTGCTGGGAG ATCCTGTGCC AACCCCAACG TGGGCTTCCA GAGACAGCTC CAGGAGTTTG 481 AGAAGCATGA GGTCCATCAG TATCGGCAGT GGCTGAAGGA AGAATATGGA GAGAGCCCTT 541 TGCAGGATGC AGAAGAAGCC AAAAACATTC TGGCCGCTCC AGGAATTCTG AAGTTCTGGG 601 CCTTTCTCAG AAGACTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGGGGAC ACCACATGGA 781 TCACCATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt