Construct: ORF TRCN0000471137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007889.1_s317c1
- Derived from:
- ccsbBroadEn_04120
- DNA Barcode:
- CCTCCCCTATAAGAAAGGCACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GEMIN7 (79760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471137
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001007269.2 | 100% | 100% | |
| 2 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001007270.2 | 100% | 100% | |
| 3 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001319054.1 | 100% | 100% | |
| 4 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_001319055.2 | 100% | 100% | |
| 5 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | NM_024707.3 | 100% | 100% | |
| 6 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | XM_005259262.3 | 100% | 100% | |
| 7 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | XM_005259263.5 | 100% | 100% | |
| 8 | human | 79760 | GEMIN7 | gem nuclear organelle assoc... | XM_017027312.1 | 100% | 100% | |
| 9 | mouse | 69731 | Gemin7 | gem nuclear organelle assoc... | NM_027189.2 | 78.1% | 80.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 483
- ORF length:
- 393
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCAAACTCC AGTGAACATT CCCGTGCCTG 121 TGCTCCGGCT GCCCCGGGGC CCTGATGGCT TCAGCCGTGG CTTTGCCCCT GATGGACGCA 181 GAGCCCCCTT GAGGCCAGAG GTTCCTGAAA TCCAGGAGTG TCCCATAGCT CAAGAATCCC 241 TGGAATCCCA GGAGCAGCGG GCACGAGCCG CCCTTCGGGA GCGTTACCTC CGCAGCCTGC 301 TGGCCATGGT GGGTCATCAG GTGAGCTTCA CGTTGCACGA GGGTGTGCGT GTGGCCGCCC 361 ACTTTGGAGC CACCGACCTG GATGTGGCCA ACTTCTACGT GTCACAGCTG CAGACTCCCA 421 TAGGTGTGCA AGCAGAGGCG CTGCTCCGAT GTAGTGACAT TATTTCATAT ACCTTCAAGC 481 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGACC TCCCCTATAA GAAAGGCACT CAACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt