Construct: ORF TRCN0000471143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003405.1_s317c1
- Derived from:
- ccsbBroadEn_14424
- DNA Barcode:
- TTCTTACTATCCCATGCCCGGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CXorf22 (170063)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471143
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286464 | CFAP47 | cilia and flagella associat... | NM_152632.4 | 76.9% | 76.7% | (many diffs) |
| 2 | human | 286464 | CFAP47 | cilia and flagella associat... | XM_017029453.1 | 39% | 38.8% | (many diffs) |
| 3 | human | 286464 | CFAP47 | cilia and flagella associat... | XM_017029452.1 | 28.2% | 28.1% | (many diffs) |
| 4 | human | 286464 | CFAP47 | cilia and flagella associat... | NM_001304548.2 | 23.5% | 23.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2328
- ORF length:
- 2262
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cacccaaaag ggttccctca ccataaacgt ccacagaggt tccctcgcca 121 tgagcatcca aaggggttcc ctcgtccccc gggatatgga tagctcgggt agagacatgc 181 agctgcgggt gatcccggct gaggtgaagt tcctggacac gatggccggg agggtgtacc 241 gcctcccgat tactgtgcat aatatttgcc gctggaacca gaaaatccga tttaaggagc 301 ccgtcaagcc acagttcaaa ctgatgttga ccagtctgga taaagaactt gcttctggcc 361 ttcagatgac agctatggtg gaatatcatc ctgataaaga cgaagacact tttgaccggc 421 tacttatttc aatagaaaat aaaacaacag aaattcctct aattgggttg attccatcct 481 gtcaattgga aattgaatca gtagttaatt ttggcacact ggttgccaat agtaaagtat 541 attctaaaga gattactatc actaaccatg gcaaagctcc aggcatattt aaggcagaat 601 accacggcca attacccatc ctcatttttc caactagtgg tatcgtggat gctaagtcat 661 caatggttat taaagtagat ttctgtgcag accagccaag aattgtagat gaagaggcaa 721 tagtgatttt gcaaggtcaa cctgagatgc tcttgagtat caaagctcat gtggttgagc 781 agattattga attattaagc atgagtagtg acagaaggct ggaatgcata cactttggtc 841 ctgttttctt cggatcatca aaaattaaac atgcacgtgt atacaataat agcccagagc 901 ccataaattg ggtggccatc atacaagatg atgccgtggg agaagaattg ggtacagata 961 ttcaacaaag aacagatatt gctttaaata atctcaccta cataagaaaa ataaagaaca 1021 tagatactac tatcattatc tcctgtcttc ctaatgaagg gactttacaa ccttatcaaa 1081 agactgtaat tacattttgt ttcaccccaa agctaatggc tgttggtaaa aaggatattg 1141 gaccttcata cagacaggac tatgctctct ttttgagatt tgagtccgta ggaagtaaag 1201 atggattttt gagagatgat gactataaaa ccatcaaaag tgaacgattt cagaaagtgg 1261 aattagcact gacaggcaca ggacttcctg ttttactaca gtttgatcca ggaccagttc 1321 ttaattttaa accttgtttc atgggtgaac gttcagaaat tcagtgcatc ataaaaaatc 1381 aatgcgaatt acttcctgtg acgtaccact ttaaaaaaac tgcaaatttt gaaattgatc 1441 ctgaaaaggg caagattact ggagggggta tggtggatgt gatgtgttca tttgttccac 1501 atcaacttgg agtcttcaaa gtgaagcaga tgatagagat tattggttta gtggcagaag 1561 aagatttgca atctttgtcg gtaaaatctt tccatcacgt atatttagct ttcaacagca 1621 tctgtaaagc ttccaccaag aaagttgtga tgaaatttga tcctggtata ttgccttcga 1681 tccgtaatcc cacgggaaag tttgtggtca aagacttggc aaaacgcaag aattatgcac 1741 ctgtagcaat gcttcaatca gccatgacac gcactcacaa tcatcgctca tgtgaagagc 1801 cagtgaagga tatgctatta gcctttccca atgaccgagc tgcaactatc aggtctaaag 1861 accatcataa aCATTTCAGG CCAATTTTCA CAAAAGTTCC AAGATTTAAC TATGTGAATC 1921 ATGATTTTGC ATATACTACA TTTGAAAAAC AGCAAAAGAA ATTATATGAA AACTATTATG 1981 CAATGTATCT TAAATATTTA AGAAGTGTGC GCTTGCAGAA GAAACAAGCA GAGAGGGAGC 2041 GCATGTATTC ATATGATGAT ACAGACATAG GCTTAGAGCC GGGATCAGGT CTAAAGTCAC 2101 CCTCACTCTC AGAAGCGGAA ATAGAAGAGG AGCTGTCTTC AGCAGCAAAT TCAATTAGAG 2161 CGAATCGATT GTTAACCACC AGGGGTATAG CATCTCAGGA GGAAGAGTCT GTGAGAAGAA 2221 AGGTTCTCAA AGGACTTAAA TCAGAACCAT CCACTCCACA AGAAAAACAT GATTGCAGCT 2281 TAATGTTGAC ACCAAAGCAA ATTCATCAAG TAAATGTTGT TAGATACTGC CCAACTTTCT 2341 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2401 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2461 GTGGAAAGGA CGATTCTTAC TATCCCATGC CCGGGTTACG CGTTAAGTCg acaatcaacc 2521 tctggattac aaaatttgtg aaagatt