Construct: ORF TRCN0000471160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001113.1_s317c1
- Derived from:
- ccsbBroadEn_07788
- DNA Barcode:
- CCATGGCCCAACTCTGGGTTATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAN1B1 (11253)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11253 | MAN1B1 | mannosidase alpha class 1B ... | NM_016219.5 | 99.9% | 100% | 2046T>C |
| 2 | human | 11253 | MAN1B1 | mannosidase alpha class 1B ... | XM_024447403.1 | 92.6% | 75.5% | 1763_1845del;2129T>C;2181_2262del |
| 3 | human | 11253 | MAN1B1 | mannosidase alpha class 1B ... | XM_006716945.4 | 88.6% | 87.1% | (many diffs) |
| 4 | human | 11253 | MAN1B1 | mannosidase alpha class 1B ... | NR_045720.2 | 76.5% | (many diffs) | |
| 5 | human | 11253 | MAN1B1 | mannosidase alpha class 1B ... | XM_017014239.1 | 75.1% | 72.2% | (many diffs) |
| 6 | human | 11253 | MAN1B1 | mannosidase alpha class 1B ... | NR_045721.2 | 73.8% | (many diffs) | |
| 7 | human | 11253 | MAN1B1 | mannosidase alpha class 1B ... | XR_001746176.1 | 71.8% | (many diffs) | |
| 8 | mouse | 227619 | Man1b1 | mannosidase, alpha, class 1... | XM_011239058.2 | 47.9% | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2163
- ORF length:
- 2097
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcctgcgag ggcaggagaa gcggagctct cggttcctct cagtcggact 121 tcctgacgcc gccagtgggc ggggcccctt gggccgtcgc caccactgta gtcatgtacc 181 caccgccgcc gccgccgcct catcgggact tcatctcggt gacgctgagc tttggcgaga 241 actatgacaa cagcaagagt tggcggcggc gctcgtgctg gaggaaatgg aagcaactgt 301 cgagattgca gcggaatatg attctcttcc tccttgcctt tctgcttttc tgtggactcc 361 tcttctacat caacttggct gaccattgga aagctctggc tttcaggcta gaggaagagc 421 agaagatgag gccagaaatt gctgggttaa aaccagcaaa tccacccgtc ttaccagctc 481 ctcagaaggc ggacaccgac cctgagaact tacctgagat ttcgtcacag aagacacaaa 541 gacacatcca gcggggacca cctcacctgc agattagacc cccaagccaa gacctgaagg 601 atgggaccca ggaggaggcc acaaaaaggc aagaagcccc tgtggatccc cgcccggaag 661 gagatccgca gaggacagtc atcagctgga ggggagcggt gatcgagcct gagcagggca 721 ccgagctccc ttcaagaaga gcagaagtgc ccaccaagcc tcccctgcca ccggccagga 781 cacagggcac accagtgcat ctgaactatc gccagaaggg cgtgattgac gtcttcctgc 841 atgcatggaa aggataccgc aagtttgcat ggggccatga cgagctgaag cctgtgtcca 901 ggtccttcag tgagtggttt ggcctcggtc tcacactgat cgacgcgctg gacaccatgt 961 ggatcttggg tctgaggaaa gaatttgagg aagccaggaa gtgggtgtcg aagaagttac 1021 actttgaaaa ggacgtggac gtcaacctgt ttgagagcac gatccgcatc ctgggggggc 1081 tcctgagtgc ctaccacctg tctggggaca gcctcttcct gaggaaagct gaggattttg 1141 gaaatcggct aatgcctgcc ttcagaacac catccaagat tccttactcg gatgtgaaca 1201 tcggtactgg agttgcccac ccgccacggt ggacctccga cagcactgtg gccgaggtga 1261 ccagcattca gctggagttc cgggagctct cccgtctcac aggggataag aagtttcagg 1321 aggcagtgga gaaggtgaca cagcacatcc acggcctgtc tgggaagaag gatgggctgg 1381 tgcccatgtt catcaatacc cacagtggcc tcttcaccca cctgggcgta ttcacgctgg 1441 gcgccagggc cgacagctac tatgagtacc tgctgaagca gtggatccag ggcgggaagc 1501 aggagacaca gctgctggaa gactacgtgg aagccatcga gggtgtcaga acgcacctgc 1561 tgcggcactc cgagcccagt aagctcacct ttgtggggga gcttgcccac ggccgcttca 1621 gtgccaagat ggaccacctg gtgtgcttcc tgccagggac gctggctctg ggcgtctacc 1681 acggccTGCC CGCCAGCCAC ATGGAGCTGG CCCAGGAGCT CATGGAGACT TGTTACCAGA 1741 TGAACCGGCA GATGGAGACG GGGCTGAGTC CCGAGATCGT GCACTTCAAC CTTTACCCCC 1801 AGCCGGGCCG TCGGGACGTG GAGGTCAAGC CAGCAGACAG GCACAACCTG CTGCGGCCAG 1861 AGACCGTGGA GAGCCTGTTC TACCTGTACC GCGTCACAGG GGACCGCAAA TACCAGGACT 1921 GGGGCTGGGA GATTCTGCAG AGCTTCAGCC GATTCACACG GGTCCCCTCG GGTGGCTATT 1981 CTTCCATCAA CAATGTCCAG GATCCTCAGA AGCCCGAGCC TAGGGACAAG ATGGAGAGCT 2041 TCTTCCTGGG GGAGACGCTC AAGTATCTGT TCTTGCTCTT CTCCGATGAC CCAAACCTGC 2101 TCAGCCTGGA CGCCTACGTG TTCAACACCG AAGCCCACCC TCTGCCTATC TGGACCCCTG 2161 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2221 TCGGTCTCGA TTCTACGTAG TAATGAACTA GCCCGTAACT TGAAAGTATT TCGATTTCTT 2281 GGCTTTATAT ATCTTGTGGA AAGGACGACC ATGGCCCAAC TCTGGGTTAT TAACGCGTTA 2341 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt