Construct: ORF TRCN0000471161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005777.1_s317c1
- Derived from:
- ccsbBroadEn_11472
- DNA Barcode:
- GATCATACTATATTTAACTTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD96 (10225)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471161
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10225 | CD96 | CD96 molecule | NM_001318889.2 | 99.9% | 100% | 816T>C |
| 2 | human | 10225 | CD96 | CD96 molecule | XM_005247063.3 | 94.6% | 94.2% | (many diffs) |
| 3 | human | 10225 | CD96 | CD96 molecule | XM_006713470.3 | 69.9% | 68.5% | (many diffs) |
| 4 | human | 10225 | CD96 | CD96 molecule | NM_005816.5 | 69.8% | 68.4% | (many diffs) |
| 5 | human | 10225 | CD96 | CD96 molecule | XM_006713469.3 | 68% | 66.6% | (many diffs) |
| 6 | human | 10225 | CD96 | CD96 molecule | NM_198196.3 | 67.9% | 66.5% | (many diffs) |
| 7 | human | 10225 | CD96 | CD96 molecule | XM_017005521.1 | 57.1% | 55.9% | (many diffs) |
| 8 | human | 10225 | CD96 | CD96 molecule | XM_017005522.1 | 54.4% | 53.1% | (many diffs) |
| 9 | human | 10225 | CD96 | CD96 molecule | NR_134917.2 | 52.2% | (many diffs) | |
| 10 | human | 10225 | CD96 | CD96 molecule | XR_241462.1 | 49.4% | (many diffs) | |
| 11 | human | 10225 | CD96 | CD96 molecule | XR_924090.1 | 47% | (many diffs) | |
| 12 | human | 10225 | CD96 | CD96 molecule | XR_001739977.1 | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1272
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaaaaatgg aaatactgtg ctgtctatta catcatccag atacattttg 121 tcaagggagt ttgggaaaaa acagtcaaca cagaagaaaa tgtttatgct acacttggct 181 ctgatgtcaa cctgacctgc caaacacaga cagtaggctt cttcgtgcag atgcaatggt 241 ccaaggtcac caataagata gacctgattg ctgtctatca tccccaatac ggcttctact 301 gtgcctatgg gagaccctgt gagtcacttg tgactttcac agaaactcct gagaatgggt 361 caaaatggac tctgcactta aggaatatgt cttgttcagt cagtggaagg tacgagtgta 421 tgcttgttct gtatccagag ggcattcaga ctaaaatcta caaccttctc attcagacac 481 acgttacagc agatgaatgg aacagcaacc atacgataga aatagagata aatcagactc 541 tggaaatacc atgctttcaa aatagctcct caaaaatttc atctgagttc acctatgcat 601 ggtcggtgga ggataatgga actcaggaaa cacttatctc ccaaaatcac ctcatcagca 661 attccacatt acttaaagat agagtcaagc ttggtacaga ctacagactc cacctctctc 721 cagtccaaat cttcgatgat gggcggaagt tctcttgcca cattagagtc ggtcctaaca 781 aaatcttgag gagctccacc acagtcaagg tttttgctaa aCCAGAAATC CCTGTGATTG 841 TGGAAAATAA CTCCACGGAT GTCTTGGTAG AGAGAAGATT CACCTGCTTA CTAAAGAATG 901 TATTTCCCAA AGCAAATATC ACATGGTTTA TAGATGGAAG TTTTCTTCAT GATGAAAAAG 961 AAGGAATATA TATTACTAAT GAAGAGAGAA AAGGCAAAGA TGGATTTTTG GAACTGAAGT 1021 CTGTTTTAAC AAGGGTACAT AGTAATAAAC CAGCCCAATC AGACAACTTG ACCATTTGGT 1081 GTATGGCTCT GTCTCCAGTC CCAGGAAATA AAGTGTGGAA CATCTCATCA GAAAAGATCA 1141 CTTTTCTCTT AGGTTCTGAA ATTTCCTCAA CAGACCCTCC ACTGAGTGTT ACAGAATCTA 1201 CCCTTGACAC CCAACCTTCT CCAGCCAGCA GTGTATCTCC TGCAAGTAAG AATGTTTTCA 1261 CACTGAGCTA TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAT CATACTATAT TTAACTTTTT 1441 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t