Transcript: Human NM_005816.5

Homo sapiens CD96 molecule (CD96), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
CD96 (10225)
Length:
4328
CDS:
53..1762

Additional Resources:

NCBI RefSeq record:
NM_005816.5
NBCI Gene record:
CD96 (10225)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005816.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230303 AGTGGAAGGTACGAGTGTATG pLKO_005 389 CDS 100% 10.800 15.120 N CD96 n/a
2 TRCN0000218889 TGTCAAGGAACTAAGGTATTT pLKO_005 3886 3UTR 100% 13.200 10.560 N CD96 n/a
3 TRCN0000218594 ACATAGCGACAGATAACTATA pLKO_005 4136 3UTR 100% 13.200 9.240 N CD96 n/a
4 TRCN0000218890 CAGCAATTCCACATTACTTAA pLKO_005 643 CDS 100% 13.200 9.240 N CD96 n/a
5 TRCN0000230304 ACGTCCATATCACTGGTATTG pLKO_005 1515 CDS 100% 10.800 7.560 N CD96 n/a
6 TRCN0000057684 CCAGTGATTGTAGCAGCTTTA pLKO.1 1568 CDS 100% 10.800 7.560 N CD96 n/a
7 TRCN0000057683 CCTGCTTACTAAAGAATGTAT pLKO.1 870 CDS 100% 5.625 3.938 N CD96 n/a
8 TRCN0000057687 CCAACGAAAGTGATCTGCCTT pLKO.1 1719 CDS 100% 2.640 1.848 N CD96 n/a
9 TRCN0000057685 CCCAGGAAATAAAGTGTGGAA pLKO.1 1087 CDS 100% 2.640 1.848 N CD96 n/a
10 TRCN0000057686 CCTGTGAGTCACTTGTGACTT pLKO.1 303 CDS 100% 0.495 0.347 N CD96 n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3576 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3577 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005816.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11472 pDONR223 100% 69.8% 68.4% None (many diffs) n/a
2 ccsbBroad304_11472 pLX_304 0% 69.8% 68.4% V5 (many diffs) n/a
3 TRCN0000471161 GATCATACTATATTTAACTTTTTA pLX_317 37.6% 69.8% 68.4% V5 (many diffs) n/a
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