Construct: ORF TRCN0000471172
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002937.1_s317c1
- Derived from:
- ccsbBroadEn_04377
- DNA Barcode:
- TTGGGTTGGATTCACCACCTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SARNP (84324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471172
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84324 | SARNP | SAP domain containing ribon... | NM_033082.4 | 100% | 100% | |
| 2 | human | 84324 | SARNP | SAP domain containing ribon... | NR_026723.1 | 52.7% | 1_55del;686_1195del | |
| 3 | human | 84324 | SARNP | SAP domain containing ribon... | NR_026722.1 | 48.7% | 1_55del;190_191ins47;639_1148del | |
| 4 | mouse | 66118 | Sarnp | SAP domain containing ribon... | NM_025364.2 | 89.5% | 95.7% | (many diffs) |
| 5 | mouse | 66118 | Sarnp | SAP domain containing ribon... | XM_006513949.3 | 89.2% | 95.2% | (many diffs) |
| 6 | mouse | 66118 | Sarnp | SAP domain containing ribon... | XM_006513950.3 | 79.6% | 84.7% | (many diffs) |
| 7 | mouse | 66118 | Sarnp | SAP domain containing ribon... | XM_006513951.3 | 79.3% | 84.2% | (many diffs) |
| 8 | mouse | 66118 | Sarnp | SAP domain containing ribon... | XM_011243523.2 | 53.9% | 58.5% | (many diffs) |
| 9 | mouse | 66118 | Sarnp | SAP domain containing ribon... | XM_011243524.2 | 53.9% | 58.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccgagacg gtggagctcc ataagctaaa gcttgccgaa ctaaagcaag 121 aatgtcttgc tcgtggtttg gagaccaagg gaataaagca agatcttatc cacagactcc 181 aggcatatct tgaagaacat gctgaagagg aggcaaatga agaagatgta ctgGGAGATG 241 AAACAGAGGA AGAAGAAACA AAGCCCATTG AGCTCCCTGT CAAAGAGGAA GAACCCCCTG 301 AAAAAACTGT TGATGTGGCA GCAGAGAAGA AAGTGGTGAA AATTACATCT GAAATACCAC 361 AGACTGAGAG AATGCAGAAG AGGGCTGAAC GATTCAATGT ACCTGTGAGC TTGGAGAGTA 421 AGAAAGCTGC TCGGGCAGCT AGGTTTGGGA TTTCTTCAGT TCCAACAAAA GGTCTGTCAT 481 CTGATAACAA ACCTATGGTT AACTTGGATA AGCTGAAGGA AAGAGCTCAA AGATTTGGTT 541 TGAATGTCTC TTCAATCTCC AGAAAGTCTG AAGATGATGA GAAACTGAAA AAGAGGAAGG 601 AGCGATTTGG GATTGTCACA AGTTCAGCTG GAACTGGAAC CACAGAGGAT ACAGAGGCAA 661 AGAAGAGGAA AAGAGCAGAG CGCTTTGGGA TTGCCTGCCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 ATTGGGTTGG ATTCACCACC TGCCAACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt