Construct: ORF TRCN0000471173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012704.1_s317c1
- Derived from:
- ccsbBroadEn_03201
- DNA Barcode:
- TCGAATAAAACCCTCCCTTTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLLP (51090)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471173
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51090 | PLLP | plasmolipin | NM_015993.3 | 100% | 100% | |
| 2 | mouse | 67801 | Pllp | plasma membrane proteolipid | NM_026385.4 | 87% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatggc cgagttcccg tcgaaagtta gcacgcggac cagcagtcct gcgcagggcg 121 ccgaagcctc ggtgtcggcg ctgcgcccgg acctgggctt cgtgcgcTCC CGCCTCGGGG 181 CGCTCATGCT GCTGCAGCTG GTGCTGGGGC TGCTGGTGTG GGCGCTGATT GCGGACACCC 241 CGTACCACCT GTATCCGGCC TATGGCTGGG TGATGTTCGT CGCTGTCTTC CTCTGGCTGG 301 TGACAATCGT CCTCTTCAAC CTCTACCTGT TTCAGCTGCA CATGAAGTTG TACATGGTTC 361 CCTGGCCACT GGTGTTAATG ATCTTTAACA TCAGCGCCAC CGTTCTCTAC ATCACCGCCT 421 TCATCGCCTG CTCTGCGGCA GTTGACCTGA CATCCCTGAG GGGCACCCGG CCTTATAACC 481 AGCGCGCGGC TGCCTCGTTC TTTGCGTGTT TGGTGATGAT CGCCTATGGA GTGAGTGCCT 541 TCTTCAGCTA CCAGGCCTGG CGAGGAGTAG GCAGCAATGC GGCCACCAGT CAGATGGCTG 601 GCGGCTATGC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCG AATAAAACCC TCCCTTTAAC 781 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t