Construct: ORF TRCN0000471180
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002197.1_s317c1
- Derived from:
- ccsbBroadEn_13154
- DNA Barcode:
- ACCCTAGACTGGACCGGGACCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1S3 (130340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471180
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130340 | AP1S3 | adaptor related protein com... | NM_001039569.2 | 90.9% | 87.1% | (many diffs) |
| 2 | human | 130340 | AP1S3 | adaptor related protein com... | XM_011510600.3 | 63.6% | 60.9% | (many diffs) |
| 3 | human | 130340 | AP1S3 | adaptor related protein com... | NR_110905.2 | 11.4% | (many diffs) | |
| 4 | human | 130340 | AP1S3 | adaptor related protein com... | NR_110906.2 | 8.8% | (many diffs) | |
| 5 | mouse | 252903 | Ap1s3 | adaptor-related protein com... | NM_183027.2 | 82.9% | 82.9% | (many diffs) |
| 6 | mouse | 252903 | Ap1s3 | adaptor-related protein com... | XR_373542.1 | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat acatttcata ttgctcttca gtcgacaagg gaaattacgg ctacagaaat 121 ggtacatcac tctccctgat aaagagagga agaagaTCAC CCGGGAAATT GTTCAGATTA 181 TTCTCTCCCG TGGTCACAGG ACAAGCAGTT TTGTTGACTG GAAGGAGCTA AAACTTGTTT 241 ATAAAAGGTA TGCTAGTTTA TATTTTTGCT GTGCAATAGA AAATCAGGAC AATGAGCTCT 301 TGACGCTAGA GATTGTGCAT CGTTACGTGG AGCTGCTGGA CAAATATTTT GGAAATGTCT 361 GTGAGCTGGA TATTATCTTT AATTTTGAAA AGGCTTATTT CATCCTGGAC GAGTTTATAA 421 TAGGTGGGGA AATTCAGGAA ACATCCAAGA AAATTGCTGT CAAAGCCATT GAAGACTCTG 481 ATATGTTACA GGAGAATCGT TTGAGCCCCA GAGGCAGAGA TTGCAGTGAG CCGAGATCAT 541 GCCATTGCAC TCTAGCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCCTAG ACTGGACCGG 721 GACCCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt