Construct: ORF TRCN0000471202
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005712.1_s317c1
- Derived from:
- ccsbBroadEn_06162
- DNA Barcode:
- GCTCCTATGGGACACACGCGCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENO3 (2027)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471202
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2027 | ENO3 | enolase 3 | NM_001976.5 | 99.8% | 99.5% | 212A>G;254T>C |
| 2 | human | 2027 | ENO3 | enolase 3 | NM_053013.4 | 99.8% | 99.5% | 212A>G;254T>C |
| 3 | human | 2027 | ENO3 | enolase 3 | XM_011523729.1 | 99.8% | 99.5% | 212A>G;254T>C |
| 4 | human | 2027 | ENO3 | enolase 3 | XM_017024346.2 | 99.8% | 99.5% | 212A>G;254T>C |
| 5 | human | 2027 | ENO3 | enolase 3 | XM_005256521.2 | 97.8% | 97.5% | 1_27del;239A>G;281T>C |
| 6 | human | 2027 | ENO3 | enolase 3 | NM_001193503.2 | 90% | 89.8% | 181_182ins129 |
| 7 | mouse | 13808 | Eno3 | enolase 3, beta muscle | NM_001136062.2 | 89.6% | 97.4% | (many diffs) |
| 8 | mouse | 13808 | Eno3 | enolase 3, beta muscle | NM_001276285.1 | 89.6% | 97.4% | (many diffs) |
| 9 | mouse | 13808 | Eno3 | enolase 3, beta muscle | NM_007933.3 | 89.6% | 97.4% | (many diffs) |
| 10 | mouse | 13808 | Eno3 | enolase 3, beta muscle | XM_006532162.3 | 89.6% | 97.4% | (many diffs) |
| 11 | mouse | 13808 | Eno3 | enolase 3, beta muscle | XM_006532161.3 | 74.8% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catgcagaaa atctttgccc gggaaatctt ggactccagg ggcaacccca 121 cggtggaggt ggacctgcac acggccaagg gccgattccg agcagctgtg cccagtgggg 181 cttccacggg tatctatgag gctctggaac taagagacgg agacaaaggc cgctacctgg 241 ggaaaggagt cctgaaggct gtggagaaca tcaacagtac tctgggccct gctctgctgc 301 aaaagaaact aagcgttgcg gatcaagaaa aagttgacaa atttatgatt gagctagatg 361 ggaccgagaa taagtccaag tttggggcca atgccatcct gggcgtgtcc ttggccgtgt 421 gtaaggcggg agcagctgag aagggggtcc ccctgtaccg ccacatcgca gatctcgctg 481 ggaaccctga cctcatactc ccagtgccag ccttcaatgt gatcaacggg ggctcccatg 541 ctggaaacaa gctggccatg caggagttca tgattctgcc tgtgggagcc agctccttca 601 aggaagccat gcgcattggc gccgaggtct accaccacct caagggggtc atcaaggcca 661 agtatgggaa ggatgccacc aatgtgggtg atgaaggtgg cttcgcaccc aacatcctgg 721 agaacaatga ggccctggag ctgctgaaga cggccatcca ggcggctggt tacccagaca 781 aggtggtgat cggcatggat gtggcagcat ctgagttcta tcgcaatggg aagtacgatc 841 ttgacttcaa gtcgccTGAT GATCCCGCAC GGCACATCAC TGGGGAGAAG CTCGGAGAGC 901 TGTATAAGAG CTTTATCAAG AACTATCCTG TGGTCTCCAT CGAAGACCCC TTTGACCAGG 961 ATGACTGGGC CACTTGGACC TCCTTCCTCT CGGGGGTGAA CATCCAGATT GTGGGGGATG 1021 ACTTGACAGT CACCAACCCC AAGAGGATTG CCCAGGCCGT TGAGAAGAAG GCCTGCAACT 1081 GTCTGCTGCT GAAGGTCAAC CAGATCGGCT CGGTGACCGA ATCGATCCAG GCGTGCAAAC 1141 TGGCTCAGTC TAATGGCTGG GGGGTGATGG TGAGCCACCG CTCTGGGGAG ACTGAGGACA 1201 CATTCATTGC TGACCTTGTG GTGGGGCTCT GCACAGGACA GATCAAGACT GGCGCCCCCT 1261 GCCGCTCGGA GCGTCTGGCC AAATACAACC AACTCATGAG GATCGAGGAG GCTCTTGGGG 1321 ACAAGGCAAT CTTTGCTGGA CGCAAGTTCC GTAACCCGAA GGCCAAGTGC CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGAGCTCCTA TGGGACACAC GCGCAGTACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt