Construct: ORF TRCN0000471209
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001281.1_s317c1
- Derived from:
- ccsbBroadEn_09014
- DNA Barcode:
- TAGAACCACACTTCCGCCCACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TM2D3 (80213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471209
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_025141.3 | 99.6% | 99.5% | 17T>G;25A>C |
| 2 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_078474.3 | 89.2% | 89% | 17T>G;25A>C;91_168del |
| 3 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_001307960.1 | 80.5% | 72.7% | (many diffs) |
| 4 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_001308026.1 | 72.1% | 65.3% | (many diffs) |
| 5 | mouse | 68634 | Tm2d3 | TM2 domain containing 3 | NM_026795.3 | 80.5% | 83.9% | (many diffs) |
| 6 | mouse | 68634 | Tm2d3 | TM2 domain containing 3 | NM_178056.3 | 71% | 73.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggaggggtg cgcccgctgc ggggcctccg cgccttgtgt cgcgtgctgc 121 tcttcctctc gcagttctgc attctgtcgg gcggtgaaag tactgaaatc ccaccttatg 181 tgatgaagtg tccgagcaat ggtttgtgta gcaggcttcc tgcagactgt atagactgca 241 caacaaattt ctcctgtacc tatgggaagc ctgtcacttt tGACTGTGCA GTGAAACCAT 301 CTGTTACCTG TGTTGATCAA GACTTCAAAT CCCAAAAGAA CTTCATCATT AACATGACTT 361 GCAGATTTTG CTGGCAGCTT CCTGAAACAG ATTACGAGTG TACCAACTCC ACCAGCTGCA 421 TGACGGTGTC CTGTCCTCGG CAGCGCTACC CTGCCAACTG CACGGTGCGG GACCACGTCC 481 ACTGCTTGGG TAACCGTACT TTTCCCAAAA TGCTATATTG CAATTGGACT GGAGGCTATA 541 AGTGGTCTAC GGCTCTGGCT CTAAGCATCA CCCTCGGTGG GTTTGGAGCA GACCGTTTCT 601 ACCTGGGCCA GTGGCGGGAA GGCCTCGGCA AGCTCTTCAG CTTCGGTGGC CTGGGAATAT 661 GGACGCTGAT AGACGTCCTG CTCATTGGAG TTGGCTATGT TGGACCAGCA GATGGCTCTT 721 TGTACATTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAGAAC CACACTTCCG CCCACACCAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt