Construct: ORF TRCN0000471213
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009048.1_s317c1
- Derived from:
- ccsbBroadEn_06634
- DNA Barcode:
- GCCGAAGGAGTGCTTTTTACAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFATC1 (4772)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471213
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_172390.3 | 99.9% | 100% | 786T>G;1986A>G |
| 2 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_001278670.2 | 97.2% | 97.4% | (many diffs) |
| 3 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_001278675.2 | 95.9% | 94.1% | (many diffs) |
| 4 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_001278672.2 | 93.3% | 91.7% | (many diffs) |
| 5 | human | 4772 | NFATC1 | nuclear factor of activated... | XM_017025783.2 | 89.1% | 88.3% | 786T>G;1902_1903ins56;1917_1918ins175 |
| 6 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_006162.5 | 85.8% | 84.7% | (many diffs) |
| 7 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_172389.3 | 82.4% | 79.8% | (many diffs) |
| 8 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_001278669.2 | 75% | 74.1% | (many diffs) |
| 9 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_172387.3 | 72.2% | 69.9% | (many diffs) |
| 10 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_172388.3 | 28.7% | 27.5% | (many diffs) |
| 11 | human | 4772 | NFATC1 | nuclear factor of activated... | NM_001278673.1 | 25.1% | 24% | (many diffs) |
| 12 | mouse | 18018 | Nfatc1 | nuclear factor of activated... | NM_001164109.1 | 82.8% | 88% | (many diffs) |
| 13 | mouse | 18018 | Nfatc1 | nuclear factor of activated... | NM_016791.4 | 79.7% | 83.2% | (many diffs) |
| 14 | mouse | 18018 | Nfatc1 | nuclear factor of activated... | NM_198429.2 | 71.3% | 74.9% | (many diffs) |
| 15 | mouse | 18018 | Nfatc1 | nuclear factor of activated... | NM_001164111.1 | 68.7% | 70.9% | (many diffs) |
| 16 | mouse | 18018 | Nfatc1 | nuclear factor of activated... | NM_001164110.1 | 62.9% | 66% | (many diffs) |
| 17 | mouse | 18018 | Nfatc1 | nuclear factor of activated... | NM_001164112.1 | 60.6% | 62.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2217
- ORF length:
- 2148
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccaagcacc agctttccag tcccttccaa gtttccactt ggccctgcgg 121 ctgcggtctt cgggagagga gaaactttgg ggcccgcgcc gcgcgccggc ggcaccatga 181 agtcagcgga ggaagaacac tatggctatg catcctccaa cgtcagcccc gccctgccgc 241 tccccacggc gcactccacc ctgccggccc cgtgccacaa ccttcagacc tccacaccgg 301 gcatcatccc gccggcggat cacccctcgg ggtacggagc agctttggac ggtgggcccg 361 cgggctactt cctctcctcc ggccacacca ggcctgatgg ggcccctgcc ctggagagtc 421 ctcgcatcga gataacctcg tgcttgggcc tgtaccacaa caataaccag tttttccacg 481 atgtggaggt ggaagacgtc ctccctagct ccaaacggtc cccctccacg gccacgctga 541 gtctgcccag cctggaggcc tacagagacc cctcgtgcct gagcccggcc agcagcctgt 601 cctcccggag ctgcaactca gaggcctcct cctacgagtc caactactcg tacccgtacg 661 cgtcccccca gacgtcgcca tggcagtctc cctgcgtgtc tcccaagacc acggaccccg 721 aggagggctt tccccgcggg ctgggggcct gcacactgct gggttccccg cggcactccc 781 cctccacctc gccccgcgcc agcgtcactg aggagagctg gctgggtgcc cgctcctcca 841 gacccgcgtc cccgtgcaac aagaggaagt acagcctcaa cggccggcag ccgccctact 901 caccccacca ctcgcccacg ccgtccccgc acggctcccc gcgggtcagc gtgaccgacg 961 actcgtggtt gggcaacacc acccagtaca ccagctcggc catcgtggcc gccatcaacg 1021 cgctgaccac cgacagcagc ctggacctgg gagatggcgt ccctgtcaag tcccgcaaga 1081 ccaccctgga gcagccgccc tcagtggcgc tcaaggtgga gcccgtcggg gaggacctgg 1141 gcagcccccc gcccccggcc gacttcgcgc ccgaagacta ctcctctttc cagcacatca 1201 ggaagggcgg cttctgcgac cagtacctgg cggtgccgca gcacccctac cagtgggcga 1261 agcccaagcc cctgtcccct acgtcctaca tgagcccgac cctgcccgcc ctggactggc 1321 agctgccgtc ccactcaggc ccgtatgagc ttcggattga ggtgcagccc aagtcccacc 1381 accgagccca ctacgagacg gagggcagcc ggggggccgt gaaggcgtcg gccggaggac 1441 accccatcgt gcagctgcat ggctacttgg agaatgagcc gctgatgctg cagcttttca 1501 ttgggacggc ggacgaccgc ctgctgcgcc cgcacgcctt ctaccaggtg caccgcatca 1561 cagggaagac cgtgtccacc accagccacg aggccatcct ctccaacacc aaagtcctgg 1621 agatcccact cctgccggag aacagcatgc gagccgtcat tgactgtgcc ggaatcctga 1681 aactcagaaa ctccgacatt gaacttcgga aaggagagac ggacatcggg aggaagaaca 1741 cacgggtacg gctggtgttc cgcgttcacg tcccgcaacc cagcggccgc acgctgtccc 1801 tgcaggtggc ctccaacccc atcgaatgct cccagcgctc agctcaggag ctgccTCTGG 1861 TGGAGAAGCA GAGCACGGAC AGCTATCCGG TCGTGGGCGG GAAGAAGATG GTCCTGTCTG 1921 GCCACAACTT CCTGCAGGAC TCCAAGGTCA TTTTCGTGGA GAAAGCCCCA GATGGCCACC 1981 ATGTCTGGGA GATGGAAGCG AAAACTGACC GGGACCTGTG CAAGCCGAAT TCTCTGGTGG 2041 TTGAGATCCC GCCGTTTCGG AATCAGAGGA TAACCAGCCC CGTTCACGTC AGTTTCTACG 2101 TCTGCAACGG GAAGAGAAAG CGAAGCCAGT ACCAGCGTTT CACCTACCTT CCCGCCAACG 2161 GTAACGCCAT CTTTCTAACC GTAAGCCGTG AACATGAGCG CGTGGGGTGC TTTTTCTTGC 2221 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2281 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2341 ATATATCTTG TGGAAAGGAC GAGCCGAAGG AGTGCTTTTT ACAGCGACGC GTTAAGTCga 2401 caatcaacct ctggattaca aaatttgtga aagatt