Construct: ORF TRCN0000471252

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005081.4_s317c1
Derived from:
ccsbBroadEn_03917
DNA Barcode:
CTCAGGCCATGTATTGCCCTAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RBKS (64080)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471252
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64080 RBKS ribokinase NM_022128.3 100% 100%
2 human 64080 RBKS ribokinase NM_001287580.1 79.1% 79.1% 0_1ins201
3 mouse 71336 Rbks ribokinase NM_153196.1 85.3% 86% (many diffs)
4 mouse 71336 Rbks ribokinase XM_006504114.1 71.1% 71.5% (many diffs)
5 mouse 71336 Rbks ribokinase XM_006504113.3 70% 69.6% (many diffs)
6 mouse 71336 Rbks ribokinase XR_376828.2 61.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1032
ORF length:
966
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcgtctggg gaaccccaga ggcagtggca agaggaggtg gcggcggtgg
121tagtggtggg ctcctgcatg accgacctgg tcagtcttac ttctcgtttg ccaaaaactg
181gagaaaccat ccatggacat aagtttttta ttggctttgg agggaaaggt gccaaccagt
241gtgtccaagc tgctcggctt ggagcaatga cgtccatggt gtgtaaggtt ggcaaagatt
301cttttggcaa tgattatata gaaaacttaa aacagaatga tatttctaca gaatttacat
361atcagactaa agatgctgct acaggaactg cttctataat tgtcaataat gaaggccaga
421atatcattgt catagtggct ggagcaaatt tacttttgaa tacggaggat ctgagggcag
481cagccaatgt cattagcaga gccaaagtca tggtctgcca gctcgaaata actccagcaa
541cttctttgga agccctaaca atggcccgca ggagtggagt gaaaaccttg ttcaatccag
601cccctgccat tgctgacctg gatccccagt tctacacccT CTCAGATGTG TTCTGCTGCA
661ATGAAAGTGA GGCTGAGATT TTAACTGGCC TCACGGTGGG CAGCGCTGCA GATGCTGGGG
721AGGCTGCATT AGTGCTCTTG AAAAGGGGCT GCCAGGTGGT AATCATTACC TTAGGGGCTG
781AAGGATGTGT GGTGCTGTCA CAGACAGAAC CTGAGCCAAA GCACATTCCC ACAGAGAAAG
841TCAAGGCTGT GGATACCACG GGTGCTGGTG ACAGCTTTGT GGGAGCTCTG GCCTTCTACC
901TGGCTTACTA TCCAAATCTG TCCTTGGAAG ACATGCTCAA CAGATCCAAT TTCATTGCAG
961CAGTCAGTGT CCAGGCTGCA GGAACACAGT CATCTTACCC TTACAAAAAA GACCTTCCGC
1021TTACTCTGTT TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1081ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1141CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTC AGGCCATGTA TTGCCCTAGC
1201CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)