Construct: ORF TRCN0000471255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012013.1_s317c1
- Derived from:
- ccsbBroadEn_03237
- DNA Barcode:
- AAGTACATCCGCGTCACCGTCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LEF1 (51176)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51176 | LEF1 | lymphoid enhancer binding f... | NM_016269.5 | 100% | 100% | |
| 2 | human | 51176 | LEF1 | lymphoid enhancer binding f... | XM_005263046.3 | 94.1% | 94.2% | (many diffs) |
| 3 | human | 51176 | LEF1 | lymphoid enhancer binding f... | NM_001130713.2 | 92.9% | 92.9% | 637_638ins84 |
| 4 | human | 51176 | LEF1 | lymphoid enhancer binding f... | NM_001130714.2 | 87.4% | 87.4% | (many diffs) |
| 5 | human | 51176 | LEF1 | lymphoid enhancer binding f... | XM_005263047.1 | 77.7% | 77.3% | (many diffs) |
| 6 | human | 51176 | LEF1 | lymphoid enhancer binding f... | NM_001166119.1 | 75.8% | 75.4% | (many diffs) |
| 7 | human | 51176 | LEF1 | lymphoid enhancer binding f... | XM_005263048.1 | 71% | 70.6% | (many diffs) |
| 8 | human | 51176 | LEF1 | lymphoid enhancer binding f... | XM_006714233.1 | 66.6% | 66.5% | (many diffs) |
| 9 | mouse | 16842 | Lef1 | lymphoid enhancer binding f... | NM_010703.4 | 91.3% | 97.2% | (many diffs) |
| 10 | mouse | 16842 | Lef1 | lymphoid enhancer binding f... | XM_006501068.3 | 86.2% | 91.5% | (many diffs) |
| 11 | mouse | 16842 | Lef1 | lymphoid enhancer binding f... | XM_006501069.2 | 84.4% | 90.4% | (many diffs) |
| 12 | mouse | 16842 | Lef1 | lymphoid enhancer binding f... | NM_001276402.1 | 79.7% | 85% | (many diffs) |
| 13 | mouse | 16842 | Lef1 | lymphoid enhancer binding f... | NM_001276403.1 | 75.9% | 79.9% | (many diffs) |
| 14 | mouse | 16842 | Lef1 | lymphoid enhancer binding f... | XM_006501070.3 | 71.6% | 74.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ccaactctcc ggaggaggtg gcggcggcgg gggggacccg gaactctgcg 121 ccacggacga gatgatcccc ttcaaggacg agggcgatcc tcagaaggaa aagatcttcg 181 ccgagatcag tcatcccgaa gaggaaggcg atttagctga catcaagtct tccttggtga 241 acgagtctga aatcatcccg gccagcaacg gacacgaggt ggccagacaa gcacaaacct 301 ctcaggagcc ctaccacgac aaggccagag aacaccccga tgacggaaag catccagatg 361 gaggcctcta caacaaggga ccctcctact cgagttattc cgggtacata atgatgccaa 421 atatgaataa cgacccatac atgtcaaatg gatctctttc tccacccatc ccgagaacat 481 caaataaagt gcccgtggtg cagccatccc atgcggtcca tcctctcacc cccctcatca 541 cttacagtga cgagcacttt tctccaggat cacacccgtc acacatccca tcagatgtca 601 actccaaaca aggcatgtcc agacatcctc cagctcctga tatccctact ttttatccct 661 tgtctccggg tggtgttgga cagatcaccc cacctcttgg ctggcaaggt cagcctgtat 721 atcccatcac gggtggattc aggcaaccct acccatcctc actgtcagtc gacacttcca 781 tgtccaggtt ttcccatcat atgattcccg gtcctcctgg tccccacaca actggcatcc 841 ctcatccagc tattgtaaca cctcaggtca aacaggaaca tccccacact gacagtgacc 901 taatgcacgt gaagcctcag catgaacaga gaaaggagca ggagccaaaa agacctcaca 961 ttaagaagcc tctgaatgct tttatgttat acatgaaaga aatgagagcg aatgtcgttg 1021 ctgagtgtac tctaaaagaa agtgcagcta tcaaccagat TCTTGGCAGA AGGTGGCATG 1081 CCCTCTCCCG TGAAGAGCAG GCTAAATATT ATGAATTAGC ACGGAAAGAA AGACAGCTAC 1141 ATATGCAGCT TTATCCAGGC TGGTCTGCAA GAGACAATTA TGGTAAGAAA AAGAAGAGGA 1201 AGAGAGAGAA ACTACAGGAA TCTGCATCAG GTACAGGTCC AAGAATGACA GCTGCCTACA 1261 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGAAA GTACATCCGC GTCACCGTCC CAACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt