Construct: ORF TRCN0000471284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016807.1_s317c1
- Derived from:
- ccsbBroadEn_06471
- DNA Barcode:
- GGAGCCCATACCGCGCTTTTCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNA5 (3741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3741 | KCNA5 | potassium voltage-gated cha... | NM_002234.4 | 99.9% | 100% | 1149T>C |
| 2 | mouse | 16493 | Kcna5 | potassium voltage-gated cha... | NM_145983.2 | 82.9% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1908
- ORF length:
- 1839
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatcgcc ctggtgcccc tggagaacgg cggtgccatg accgtcagag 121 gaggcgatga ggcccgggca ggctgcggcc aggccacagg gggagagctc cagtgtcccc 181 cgacggctgg gctcagcgat gggcccaagg agccggcgcc aaaggggcgc ggcgcgcaga 241 gagacgcgga ctcgggagtg cggcccttgc ctccgctgcc ggacccggga gtgcggccct 301 tgcctccgct gccagaggag ctgccacggc ctcgacggcc gcctcccgag gacgaggagg 361 aagaaggcga tcccggcctg ggcacggtgg aggaccaggc tctgggcacg gcgtccctgc 421 accaccagcg cgtccacatc aacatctccg ggctgcgctt tgagacgcag ctgggcaccc 481 tggcgcagtt ccccaacaca ctcctggggg accccgccaa gcgcctgcgc tacttcgacc 541 ccctgaggaa cgagtacttc ttcgaccgca accggcccag cttcgacggt atcctctact 601 actaccagtc cgggggccgc ctgcggaggc cggtcaacgt ctccctggac gtgttcgcgg 661 acgagatacg cttctaccag ctgggggacg aggccatgga gcgcttccgc gaggatgagg 721 gcttcattaa agaagaggag aagcccctgc cccgcaacga gttccagcgc caggtgtggc 781 ttatcttcga gtatccggag agctctgggt ccgcgcgggc catcgccatc gtctcggtct 841 tggttatcct catctccatc atcaccttct gcttggagac cctgcctgag ttcagggatg 901 aacgtgagct gctccgccac cctccggcgc cccaccagcc tcccgcgccc gcccctgggg 961 ccaacggcag cggggtcatg gccccgccct ctggccctac ggtggcaccg ctcctgccca 1021 ggaccctggc cgaccccttc ttcatcgtgg agaccacgtg cgtcatctgg ttcaccttcg 1081 agctgctcgt gcgcttcttc gcctgcccca gcaaggcagg gttctcccgg aacatcatga 1141 acatcatcga tgtggtggcc atcttcccct acttcatcac cctgggcacc gaactggcag 1201 agcagcagcc agggggcgga ggaggcggcc agaatgggca gcaggccatg tccctggcca 1261 tcctccgagt catccgcctg gtccgggtgt tccgcatctt caagctctcc cgccactcca 1321 aggggctgca gatcctgggc aagaccttgc aggcctccat gagggagctg gggctgctca 1381 tcttcttcct cttcatcggg gtcatcctct tctccagtgc cgtctacttc gcagaggctg 1441 acaaccaggg aacccatttc tctagcatcc ctgacgcctt ctggtgggca gtggtcacca 1501 tgaccactgt gggctacggg gacatgaggC CCATCACTGT TGGGGGCAAG ATCGTGGGCT 1561 CGCTGTGTGC CATCGCCGGG GTCCTCACCA TTGCCCTGCC TGTGCCCGTC ATCGTCTCCA 1621 ACTTCAACTA CTTCTACCAC CGGGAAACGG ATCACGAGGA GCCGGCAGTC CTTAAGGAAG 1681 AGCAGGGCAC TCAGAGCCAG GGGCCGGGGC TGGACAGAGG AGTCCAGCGG AAGGTCAGCG 1741 GGAGCAGGGG ATCCTTCTGC AAGGCTGGGG GGACCCTGGA GAATGCAGAC AGTGCCCGAA 1801 GGGGCAGCTG CCCCCTAGAG AAGTGTAACG TCAAGGCCAA GAGCAACGTG GACTTGCGGA 1861 GGTCCCTTTA TGCCCTCTGC CTGGACACCA GCCGGGAAAC AGATTTGTTG CCAACTTTCT 1921 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1981 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2041 GTGGAAAGGA CGAGGAGCCC ATACCGCGCT TTTCGTAACG CGTTAAGTCg acaatcaacc 2101 tctggattac aaaatttgtg aaagatt