Transcript: Human NM_002234.4

Homo sapiens potassium voltage-gated channel subfamily A member 5 (KCNA5), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
KCNA5 (3741)
Length:
2910
CDS:
270..2111

Additional Resources:

NCBI RefSeq record:
NM_002234.4
NBCI Gene record:
KCNA5 (3741)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002234.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000431399 ACACCCAAGGGTCGCCTATTT pLKO_005 2287 3UTR 100% 13.200 18.480 N KCNA5 n/a
2 TRCN0000044163 CCTAGAGAAGTGTAACGTCAA pLKO.1 2015 CDS 100% 4.050 5.670 N KCNA5 n/a
3 TRCN0000044165 CGCGGACGAGATACGCTTCTA pLKO.1 857 CDS 100% 1.650 2.310 N KCNA5 n/a
4 TRCN0000434885 ACATTATACCGCAGAGTATTT pLKO_005 2178 3UTR 100% 13.200 9.240 N KCNA5 n/a
5 TRCN0000044164 GTGTGGCTTATCTTCGAGTAT pLKO.1 975 CDS 100% 4.950 3.465 N KCNA5 n/a
6 TRCN0000044166 GCTGACAACCAGGGAACCCAT pLKO.1 1638 CDS 100% 0.880 0.616 N KCNA5 n/a
7 TRCN0000044167 GATGAGGGCTTCATTAAAGAA pLKO.1 915 CDS 100% 5.625 3.375 N KCNA5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002234.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06471 pDONR223 100% 99.9% 100% None 1149T>C n/a
2 ccsbBroad304_06471 pLX_304 0% 99.9% 100% V5 1149T>C n/a
3 TRCN0000471284 GGAGCCCATACCGCGCTTTTCGTA pLX_317 20.8% 99.9% 100% V5 1149T>C n/a
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