Construct: ORF TRCN0000471295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016898.1_s317c1
- Derived from:
- ccsbBroadEn_05472
- DNA Barcode:
- CGAGTGACGGCATGAGGGGAGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN33 (340348)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340348 | TSPAN33 | tetraspanin 33 | NM_178562.5 | 100% | 100% | |
| 2 | human | 340348 | TSPAN33 | tetraspanin 33 | XM_006715960.3 | 99.6% | 99.6% | 158_159insAGA |
| 3 | mouse | 232670 | Tspan33 | tetraspanin 33 | NM_146173.3 | 90.6% | 96.8% | (many diffs) |
| 4 | mouse | 232670 | Tspan33 | tetraspanin 33 | NM_001301407.1 | 90.3% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 915
- ORF length:
- 849
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcggagaccc cgggcgccgg ccgcctccgg ggaggagttc tccttcgtca 121 gcccgctggt gaaatacctg ctcttcttct tcaacatgct cttctgggtg atttccatgg 181 tgatggtggc tgtgggtgtc tacgctcggc taatgaagca tgcagaagca gccctagcct 241 gcctggcagt ggaccctgcc atcctgctga tcgtggtggg tgtcctcatg ttcctgctca 301 ccttctgtgg ctgcattggg tccctccgcg agaacatctg cctcctgcag acgttctccc 361 tctgcctcac cgctgtgttc ctgctgcagc tggccgctgg gatcctgggc ttcgtcttct 421 cagacaaggc tcgagggaaa gtgagtgaga tcatcaacaa tgccattgtg cactaccgag 481 atgacttGGA TCTGCAGAAC CTCATTGATT TTGGCCAGAA AAAGTTTAGC TGCTGTGGAG 541 GGATTTCCTA CAAGGACTGG TCTCAGAACA TGTATTTCAA CTGCTCAGAA GACAACCCCA 601 GTCGAGAGCG CTGCTCTGTG CCTTACTCCT GTTGCTTGCC TACTCCTGAC CAGGCAGTGA 661 TCAACACTAT GTGTGGCCAA GGTATGCAGG CCTTTGACTA CTTGGAAGCT AGCAAAGTCA 721 TCTACACCAA TGGCTGTATT GACAAGTTGG TCAACTGGAT ACACAGCAAC CTATTCTTAC 781 TTGGTGGTGT GGCTCTAGGC CTGGCCATCC CCCAGCTGGT GGGAATTCTG CTGTCCCAGA 841 TCCTAGTGAA TCAGATCAAA GATCAGATCA AGCTACAGCT CTACAACCAG CAGCACCGGG 901 CTGACCCATG GTACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1021 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGAGTGACGG CATGAGGGGA 1081 GATGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt