Construct: ORF TRCN0000471299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007443.1_s317c1
- Derived from:
- ccsbBroadEn_08412
- DNA Barcode:
- AACGTCATTCTCGCCCCTCACAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QPCTL (54814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471299
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54814 | QPCTL | glutaminyl-peptide cyclotra... | NM_001163377.2 | 99.8% | 100% | 822G>A |
| 2 | human | 54814 | QPCTL | glutaminyl-peptide cyclotra... | NM_017659.4 | 75.3% | 75.3% | 350_631del;1104G>A |
| 3 | human | 54814 | QPCTL | glutaminyl-peptide cyclotra... | XM_011527048.3 | 61.1% | 44.4% | (many diffs) |
| 4 | human | 54814 | QPCTL | glutaminyl-peptide cyclotra... | XM_017026900.1 | 44.1% | 43.9% | 350_631del;786_787insGAGC;789_789delCins354 |
| 5 | human | 54814 | QPCTL | glutaminyl-peptide cyclotra... | XR_001753711.1 | 32.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ttccgggggc cgcgggcgac cccgcctgcg gctgggggaa cgtggcctca 121 tggagccact cttgccgccg aagcgccgcc tgctaccgcg ggttcggctc ttgcctctgt 181 tgctggcgct ggccgtgggc tcggcgttct acaccatttg gagcggctgg caccgcagga 241 ctgaggagct gccgctgggc cgggagctgc gggtcccatt gatcggaagc ctccccgaag 301 cccggctgcg gagggtggtg ggacaactgg atccacagcg tctctggagc acttatctgc 361 gccccctgct ggttgtgcga accccgggca gcccgggaaa tctccaagtc agaaaggcag 421 ccccggtgac cctgcaactg ctcttcttgg atggtgaaga ggcgctgaag gagtggggac 481 ccaaggACTC CCTTTACGGT TCCCGGCACC TGGCCCAGCT CATGGAGTCT ATACCTCACA 541 GCCCCGGCCC CACCAGGATC CAGGCTATTG AGCTCTTTAT GCTTCTTGAT CTCCTGGGAG 601 CCCCCAATCC CACCTTCTAC AGCCACTTCC CTCGCACGGT CCGCTGGTTC CATCGGCTGA 661 GGAGCATTGA GAAGCGTCTG CACCGTTTGA ACCTGCTGCA GTCTCATCCC CAGGAAGTGA 721 TGTACTTCCA ACCCGGGGAG CCCTTTGGCT CTGTGGAAGA CGACCACATC CCCTTCCTCC 781 GCAGAGGGGT ACCCGTGCTC CATCTCATCT CCACGCCCTT CCCTGCTGTC TGGCACACCC 841 CTGCGGACAC CGAGGTCAAT CTCCACCCAC CCACGGTACA CAACTTATGC CGCATTCTCG 901 CTGTGTTCCT GGCTGAATAC CTGGGGCTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACGT 1081 CATTCTCGCC CCTCACAAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt