Construct: ORF TRCN0000471300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000264.1_s317c1
- Derived from:
- ccsbBroadEn_11059
- DNA Barcode:
- AGCCACGAGTTGTGGCAACGTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC3 (5611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5611 | DNAJC3 | DnaJ heat shock protein fam... | XM_011521104.3 | 37.9% | 31.4% | (many diffs) |
| 2 | human | 5611 | DNAJC3 | DnaJ heat shock protein fam... | NM_006260.5 | 36.4% | 30% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcccccggc tccgtgacca gccggctggg ctcggtattc cccttcctgc 121 tagtcctggt ggatctgcag tacgaaggtg ctgaatgtgg agtaaatgca gatgttgaga 181 aacatcttga attgggcaag aaattacttg cagctggaca gctagctgat gctttatctc 241 agtttcatgc tgccgtagat ggtgacccTG ATAACTATAT TGCTTATTAT CGGAGGGCTA 301 CTGTCTTTTT AGCTATGGGC AAATCAAAAG CTGCACTTCC TGATTTAACT AAAGTGATTC 361 AATTGAAGAT GGACTTCACT GCAGCAAGAT TACAGAGAGG TCACTTATTA CTCAAACAAG 421 GAAAACTTGA TGAAGCAGAA GATGATTTTA AAAAAGTGGT GTTTCCTGTT CCTTCTCTGT 481 TGGGACTCCA GCGTTCTCTC CTAGATGATC TATACTTGCT ATTTTGGTTC TTCCTTATGA 541 AGAAGGTGAC TTTCAGATGC CTCTCGTCAG CCATCTCTGA ATGCCTTCCT CAGTCTTTAA 601 ATTTGATGAA GTTCAATTTG TTGATTTCCT TTTTACTTCT GTGGACTGTG CGTTTGGTGT 661 CATGTCTAAG AAGTATTCAC TACGCCGTAG GATCTAAAAC TTTTCTCATA TCTTCTAAAA 721 GTTTTATGGT TTTGTGTTTT ATTTTTAAGC CCATAGTCTA TTTGAGTTAC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGAAGCCACG AGTTGTGGCA ACGTAGGACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt