Transcript: Human NM_006260.5

Homo sapiens DnaJ heat shock protein family (Hsp40) member C3 (DNAJC3), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
DNAJC3 (5611)
Length:
5590
CDS:
118..1632

Additional Resources:

NCBI RefSeq record:
NM_006260.5
NBCI Gene record:
DNAJC3 (5611)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006260.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000146369 CAGTCGCAGAAACGAGATTAT pLKO.1 1282 CDS 100% 13.200 18.480 N DNAJC3 n/a
2 TRCN0000149107 GCTGCGTCAAAGTTGAAGAAT pLKO.1 754 CDS 100% 5.625 4.500 N DNAJC3 n/a
3 TRCN0000149046 GAGCCAAGCATTGCTGAATAT pLKO.1 1009 CDS 100% 13.200 9.240 N DNAJC3 n/a
4 TRCN0000147154 CCAGATAACTTCCAGAATGAA pLKO.1 1384 CDS 100% 5.625 3.938 N DNAJC3 n/a
5 TRCN0000148819 CACCCAGATAACTTCCAGAAT pLKO.1 1381 CDS 100% 4.950 3.465 N DNAJC3 n/a
6 TRCN0000149137 GTTCGTTCAAAGGAGAGGATT pLKO.1 1033 CDS 100% 4.950 3.465 N DNAJC3 n/a
7 TRCN0000146997 CCAGCAAATATGAATCTGTCA pLKO.1 980 CDS 100% 2.640 1.848 N DNAJC3 n/a
8 TRCN0000149484 GTGGAGATTATACTGCTGCTA pLKO.1 617 CDS 100% 2.640 1.848 N DNAJC3 n/a
9 TRCN0000150007 CATCTTGAATTGGGCAAGAAA pLKO.1 235 CDS 100% 0.563 0.394 N DNAJC3 n/a
10 TRCN0000149287 GCTAAAGAAGTCCTCTCTGAT pLKO.1 1450 CDS 100% 4.950 2.970 N DNAJC3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006260.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01293 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01293 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466430 AGCAGCAGATACCCGAAGAAGGCT pLX_317 28.9% 100% 100% V5 n/a
4 ccsbBroadEn_11059 pDONR223 100% 36.4% 30% None (many diffs) n/a
5 ccsbBroad304_11059 pLX_304 0% 36.4% 30% V5 (many diffs) n/a
6 TRCN0000471300 AGCCACGAGTTGTGGCAACGTAGG pLX_317 70.6% 36.4% 30% V5 (many diffs) n/a
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