Construct: ORF TRCN0000471301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016437.1_s317c1
- Derived from:
- ccsbBroadEn_15886
- DNA Barcode:
- TTCTACGAGTCTAGTGTCTACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC186 (55088)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55088 | CCDC186 | coiled-coil domain containi... | NM_153249.1 | 99.8% | 100% | 120T>C |
| 2 | human | 55088 | CCDC186 | coiled-coil domain containi... | XM_011539916.3 | 35.5% | 35.5% | 120T>C;634_1779del |
| 3 | human | 55088 | CCDC186 | coiled-coil domain containi... | NM_001321829.1 | 23.4% | 23.4% | 120T>C;634_2694del |
| 4 | human | 55088 | CCDC186 | coiled-coil domain containi... | NM_018017.4 | 23.4% | 23.4% | 120T>C;634_2694del |
| 5 | human | 55088 | CCDC186 | coiled-coil domain containi... | NR_135815.1 | 8% | 1_392del;512T>C;1026_7873del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agagacagac cacatagcct ctacttcctc tgataaaaat gttgggaaaa 121 cacctgaatt aaaggaagac tcatgcaact tgttttctgg caatgaaagc agcaaattag 181 aaaacgagtc caaactattg tcattaaaca ctgataaAAC TTTATGTCAA CCTAATGAGC 241 ATAATAATCG AATTGAAGCC CAGGAAAATT ATATTCCAGA TCATGGTGGA GGTGAGGATT 301 CTTGTGCCAA AACAGACACA GGCTCAGAAA ATTCTGAACA AATAGCTAAT TTTCCTAGTG 361 GAAATTTTGC TAAACATATT TCAAAAACAA ATGAAACAGA ACAGAAAGTA ACACAAATAT 421 TGGTGGAATT AAGGTCATCT ACATTTCCAG AATCAGCTAA TGAAAAGACT TATTCAGAAA 481 GCCCCTATGA TACAGACTGC ACCAAGAAAT TTATTTCAAA AATAAAGAGC GTTTCAGCAT 541 CAGAGGATTT GTTGGAAGAA ATAGAATCTG AGCTCTTATC TACGGAGTTT GCAGAACATC 601 GAGTACCAAA TGGAATGAAT AAGGGAGAAC ATGCATTAGT TCTGTTTGAA AAGTGTGTGC 661 AAGATAAATA TTTGCAGCAG GAACATATCA TAAAAAAGTA CCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGATTCTAC GAGTCTAGTG TCTACCCCAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt