Construct: ORF TRCN0000471303
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000002.1_s317c1
- Derived from:
- ccsbBroadEn_08206
- DNA Barcode:
- CATCCCTTTATTGTTACCTACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATL1 (51062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471303
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51062 | ATL1 | atlastin GTPase 1 | NM_015915.4 | 99.9% | 100% | 351G>A |
| 2 | human | 51062 | ATL1 | atlastin GTPase 1 | NM_001127713.1 | 99% | 99.1% | 351G>A;1549_1550insAGGGAAGTACAAATG |
| 3 | human | 51062 | ATL1 | atlastin GTPase 1 | NM_181598.3 | 99% | 99.1% | 351G>A;1549_1550insAGGGAAGTACAAATG |
| 4 | mouse | 73991 | Atl1 | atlastin GTPase 1 | NM_178628.5 | 90.8% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1740
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caagaaccgc agggacagaa acagttgggg tggattttcg gaaaagacat 121 atgaatggag ctcagaagag gaggagccag tgaaaaaggc aggaccagtc caagtcctca 181 ttgtcaaaga tgaccattcc tttgagttag atgaaactgc attaaatcgg atccttctct 241 cggaggctgt cagagacaag gaggttgttg ctgtatctgt tgctggagca tttagaaaag 301 gaaaatcatt cctgatggac ttcatgttga gatacatgta caaccaggaa tcagttgatt 361 gggttggaga ctacaatgaa ccattgactg gtttttcatg gagaggtgga tctgaacgag 421 agaccacagg aattcagata tggagtgaaa tcttccttat caataaacct gatggtaaaa 481 aggttgcagt gttattgatg gatactcagg gaacctttga tagtcagtca actttgagag 541 attcagccac agtatttgcc cttagcacaa tgatcagctc aatacaggta tataacttat 601 cccaaaatgt ccaggaggat gatcttcagc acctccagct tttcactgag tatggcagac 661 tggcaatgga ggaaacattc ctgaagccat ttcagagtct gatatttctt gttcgagact 721 ggagtttccc atacgaattt tcatatggag ccgatggtgg tgccaaattc ttggaaaaac 781 gcctcaaggt ctcagggaac cagcatgaag aactacagaa cgtcagaaaa cacatccatt 841 cctgtttcac caacatttcc tgttttctgc tacctcatcc tggcttaaaa gtagctacca 901 atccaaactt tgatggaaaa ttgaaagaaa tagatgatga attcatcaaa aacttgaaaa 961 tactgattcc ttggctactt agtcccgaga gcctagatat taaagagatc aatgggaata 1021 aaatcacctg ccggggtctg gtggagtact tcaaggctta tataaagatc tatcaaggtg 1081 aagaattacc acatcccaaa tccatgttac aggccacagc agaagctaac aatttagcag 1141 ccgtggcaac tgccaaggac acatacaaca aaaaaatgga agagatttgt ggtggtgaca 1201 aaccatttct ggccccaaat gacttgcaga ccaaacacct gcaacttaag gaagaatctg 1261 tgaagctatt ccgaggggtg aagaagatgg gtggggaaga atttagccgg cgttaccTGC 1321 AGCAGTTGGA GAGTGAAATA GATGAACTTT ACATCCAATA TATCAAGCAC AATGATAGCA 1381 AAAATATCTT CCATGCAGCT CGTACCCCAG CCACACTGTT TGTAGTCATC TTTATCACAT 1441 ATGTGATTGC TGGTGTGACT GGATTCATTG GTTTGGACAT CATAGCTAGC CTATGCAATA 1501 TGATAATGGG ACTGACCCTT ATCACCCTGT GCACTTGGGC ATATATCCGG TACTCTGGAG 1561 AATACCGAGA GCTGGGAGCT GTAATAGACC AGGTGGCTGC AGCTCTGTGG GACCAGGGAA 1621 GTACAAATGA GGCTTTGTAC AAGCTTTACA GTGCAGCAGC AACCCACAGA CATCTGTATC 1681 ATCAAGCTTT CCCTACACCA AAGTCGGAAT CTACTGAACA ATCAGAAAAG AAAAAAATGT 1741 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1801 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1861 TTTATATATC TTGTGGAAAG GACGACATCC CTTTATTGTT ACCTACTGCA CGCGTTAAGT 1921 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt