Construct: ORF TRCN0000471303

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000002.1_s317c1
Derived from:
ccsbBroadEn_08206
DNA Barcode:
CATCCCTTTATTGTTACCTACTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATL1 (51062)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471303
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51062 ATL1 atlastin GTPase 1 NM_015915.4 99.9% 100% 351G>A
2 human 51062 ATL1 atlastin GTPase 1 NM_001127713.1 99% 99.1% 351G>A;1549_1550insAGGGAAGTACAAATG
3 human 51062 ATL1 atlastin GTPase 1 NM_181598.3 99% 99.1% 351G>A;1549_1550insAGGGAAGTACAAATG
4 mouse 73991 Atl1 atlastin GTPase 1 NM_178628.5 90.8% 96.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1740
ORF length:
1674
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc caagaaccgc agggacagaa acagttgggg tggattttcg gaaaagacat
121atgaatggag ctcagaagag gaggagccag tgaaaaaggc aggaccagtc caagtcctca
181ttgtcaaaga tgaccattcc tttgagttag atgaaactgc attaaatcgg atccttctct
241cggaggctgt cagagacaag gaggttgttg ctgtatctgt tgctggagca tttagaaaag
301gaaaatcatt cctgatggac ttcatgttga gatacatgta caaccaggaa tcagttgatt
361gggttggaga ctacaatgaa ccattgactg gtttttcatg gagaggtgga tctgaacgag
421agaccacagg aattcagata tggagtgaaa tcttccttat caataaacct gatggtaaaa
481aggttgcagt gttattgatg gatactcagg gaacctttga tagtcagtca actttgagag
541attcagccac agtatttgcc cttagcacaa tgatcagctc aatacaggta tataacttat
601cccaaaatgt ccaggaggat gatcttcagc acctccagct tttcactgag tatggcagac
661tggcaatgga ggaaacattc ctgaagccat ttcagagtct gatatttctt gttcgagact
721ggagtttccc atacgaattt tcatatggag ccgatggtgg tgccaaattc ttggaaaaac
781gcctcaaggt ctcagggaac cagcatgaag aactacagaa cgtcagaaaa cacatccatt
841cctgtttcac caacatttcc tgttttctgc tacctcatcc tggcttaaaa gtagctacca
901atccaaactt tgatggaaaa ttgaaagaaa tagatgatga attcatcaaa aacttgaaaa
961tactgattcc ttggctactt agtcccgaga gcctagatat taaagagatc aatgggaata
1021aaatcacctg ccggggtctg gtggagtact tcaaggctta tataaagatc tatcaaggtg
1081aagaattacc acatcccaaa tccatgttac aggccacagc agaagctaac aatttagcag
1141ccgtggcaac tgccaaggac acatacaaca aaaaaatgga agagatttgt ggtggtgaca
1201aaccatttct ggccccaaat gacttgcaga ccaaacacct gcaacttaag gaagaatctg
1261tgaagctatt ccgaggggtg aagaagatgg gtggggaaga atttagccgg cgttaccTGC
1321AGCAGTTGGA GAGTGAAATA GATGAACTTT ACATCCAATA TATCAAGCAC AATGATAGCA
1381AAAATATCTT CCATGCAGCT CGTACCCCAG CCACACTGTT TGTAGTCATC TTTATCACAT
1441ATGTGATTGC TGGTGTGACT GGATTCATTG GTTTGGACAT CATAGCTAGC CTATGCAATA
1501TGATAATGGG ACTGACCCTT ATCACCCTGT GCACTTGGGC ATATATCCGG TACTCTGGAG
1561AATACCGAGA GCTGGGAGCT GTAATAGACC AGGTGGCTGC AGCTCTGTGG GACCAGGGAA
1621GTACAAATGA GGCTTTGTAC AAGCTTTACA GTGCAGCAGC AACCCACAGA CATCTGTATC
1681ATCAAGCTTT CCCTACACCA AAGTCGGAAT CTACTGAACA ATCAGAAAAG AAAAAAATGT
1741ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1801GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1861TTTATATATC TTGTGGAAAG GACGACATCC CTTTATTGTT ACCTACTGCA CGCGTTAAGT
1921Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)