Construct: ORF TRCN0000471306
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011349.1_s317c1
- Derived from:
- ccsbBroadEn_03063
- DNA Barcode:
- CATGCCTACTCTTAACTAACACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL13 (28998)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471306
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28998 | MRPL13 | mitochondrial ribosomal pro... | NM_014078.6 | 100% | 100% | |
| 2 | mouse | 68537 | Mrpl13 | mitochondrial ribosomal pro... | NM_026759.3 | 87.2% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gagtttctcT AGGGCGCCCC AGCAATGGGC CACTTTTGCT AGAATATGGT 121 ATCTCTTAGA TGGGAAAATG CAGCCACCTG GCAAACTTGC TGCTATGGCA TCTATAAGAC 181 TTCAGGGATT ACATAAACCT GTGTACCATG CACTGAGTGA CTGTGGGGAT CATGTTGTTA 241 TAATGAACAC AAGACACATT GCATTTTCTG GAAACAAATG GGAACAAAAA GTATACTCTT 301 CGCATACTGG CTACCCAGGT GGATTTAGAC AAGTAACAGC TGCTCAGCTT CACCTGAGGG 361 ATCCAGTGGC AATTGTAAAA CTAGCTATTT ATGGCATGCT GCCAAAAAAC CTTCACAGAA 421 GAACAATGAT GGAAAGGTTG CATCTTTTTC CAGATGAGTA TATTCCAGAA GATATTCTTA 481 AGAATTTAGT AGAGGAGCTT CCTCAACCAC GAAAAATACC TAAACGTCTA GATGAGTACA 541 CACAAGAAGA AATAGACGCC TTCCCAAGAT TGTGGACTCC ACCTGAAGAT TATCGGCTAT 601 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGACATGC CTACTCTTAA CTAACACAAA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt