Construct: ORF TRCN0000471329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012795.1_s317c1
- Derived from:
- ccsbBroadEn_02252
- DNA Barcode:
- AAATCCCTTTCATCGTTGACCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP11A (9824)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9824 | ARHGAP11A | Rho GTPase activating prote... | NM_199357.2 | 100% | 100% | |
| 2 | human | 114118903 | ARHGAP11A-SCG5 | ARHGAP11A-SCG5 readthrough | NM_001368319.1 | 74.5% | 63.1% | (many diffs) |
| 3 | human | 89839 | ARHGAP11B | Rho GTPase activating prote... | NM_001039841.2 | 52.7% | 44.1% | (many diffs) |
| 4 | human | 9824 | ARHGAP11A | Rho GTPase activating prote... | NM_014783.6 | 48.8% | 48.3% | (many diffs) |
| 5 | human | 89839 | ARHGAP11B | Rho GTPase activating prote... | NR_148423.1 | 45.3% | (many diffs) | |
| 6 | human | 9824 | ARHGAP11A | Rho GTPase activating prote... | NM_001286479.2 | 30.3% | 29.9% | (many diffs) |
| 7 | human | 9824 | ARHGAP11A | Rho GTPase activating prote... | NM_001286480.3 | 30.3% | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggatcagagg ctggtgaggt tggccctgtt gcagcatctg cgggccttct 121 atggtattaa ggtgaagggt gtccgtgggc agtgcgatcg caggagacat gaaacagcag 181 ccacggaaat agggggtaaa atatttggag taccttttaa tgcactgccc cattctgctg 241 taccagaata tggacacatt ccaagctttc ttgtcgatgc ttgcacatct ttagaagacc 301 atattcatac cgaagggctt tttcggaaat caggatctgt gattcgccta aaagcactaa 361 agaataaagt ggatcatggt gaaggttgcc tatcttctgc acctccttgt gatattgcgg 421 gacttcttaa gcagtttttt agggaactgc cagagcccat tctcccagct gatttgcatg 481 aagcactttt gaaagctcaa cagttaggca cagaggaaaa gaataaagct acactgttgc 541 tctcctgtct tctggctgac cacacagttc atgtattaag atacttcttt aactttctca 601 ggaatgtttc tcttagatcc agtgagaata agatggacag cagcaatctt gcagtaatat 661 ttgcaccgaa tcttcttcag acaagtgaag gacatgaaaa gatgtcttct aacacagaaa 721 agaagctacg attacaggct gcagtagtac agactcttat cgattatgca tcagatattg 781 ggcgtgtacc agattttatc ctggaaaaga taccagccat gttgggtatt gatggtctct 841 gtgctactcc atcactggaa ggctttgaag aaggtgaata tgaaactcct ggtgaatata 901 agagaaagag aagacaaagt gtaggagatt ttgttagtgg agcactaaat aaatttaaac 961 ctaacagaac accttctatt acacctcaag aagaaagaat tgcccagcta tctgaatcac 1021 cagtgattct tacaccaaat gctaagcgta cattgccagt agattcttct catggtttct 1081 caagtaagaa aaggaagtcc atcaagcaca attttaactt tgagctgttg ccaagtaatc 1141 tcttcaatag cagttctaca ccggtatcag ttcacatcga tacaagctca gaagggtcat 1201 ctcagagttC ACTCTCTCCT GTACTCATTG GTGGAAACCA TTTGATCACT GCAGGTGTGC 1261 CAAGGCGAAG TAAAAGAATT GCAGGCAAAA AAGTTTGCAG AGTGGAATCA GGAAAAGCAG 1321 GCTGCTTTTC TCCTAAAATC AGCCATAAAG AAAAGGTTCG AAGATCTCTG CGTTTGAAAT 1381 TCAATCTAGG GAAAAATGGC AGAGAAGTAA ATGGATGTTC TGGTGTCAAT AGATATGAAA 1441 GTGTTGGTTG GCGACTTGCA AATCAACAAA GTTTAAAAAA TCGAATTGAA TCTGTAAAAA 1501 CAGGTTTGCT TTTTAGCCCA GATGTTGATG AAAAGTTACC AAAGAAAGGT ACATTTACAT 1561 ACTACTGTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAATCC CTTTCATCGT TGACCCCCAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt