Construct: ORF TRCN0000471340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015307.1_s317c1
- Derived from:
- ccsbBroadEn_06090
- DNA Barcode:
- ACCAACACTTTAAGAAACATTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDOST (1650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471340
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1650 | DDOST | dolichyl-diphosphooligosacc... | NM_005216.5 | 96.1% | 96.2% | 0_1ins51;315C>T;900C>T |
| 2 | mouse | 13200 | Ddost | dolichyl-di-phosphooligosac... | NM_007838.2 | 85.2% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1434
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gtacttccgg tgtgcaggtg ctgggtcctt cggcaggagg aggaagatgg 121 agcccagcac cgcggcccgg gcttgggccc tcttttggtt gctgctgccc ttgcttggcg 181 cggtttgcgc cagcggaccc cgcaccttag tgctgctgga caacctcaac gtgcgggaga 241 ctcattcgct tttcttccgg agcctgaagg accggggctt tgagctcaca ttcaagaccg 301 ctgatgaccc cagcctgtct ctcataaagt atggggaatt cctctatgac aatctcatca 361 ttttctcccc ttcggtagaa gattttggag gcaacatcaa cgtggagacc atcagtgcct 421 ttattgacgg tggaggcagt gtgctggtag ctgccagctc cgacattggt gaccctcttc 481 gagagctggg cagtgagtgc gggattgagt ttgacgagga gaaaacggct gtcattgacc 541 atcacaacta tgacatctca gaccttggcc agcatacgct catcgtggct gacactgaga 601 acctgctgaa ggccccaacc atcgttggga aatcatctct aaatcccatc ctctttcgag 661 gtgttgggat ggtggccgat cctgataacc ctttggtgct ggacatcctg acgggctctt 721 ccacctctta ctccttcttc ccggacaagc ctatcaccca gtatccacat gcggtgggga 781 agaacaccct cctcattgct gggctccagg ccaggaacaa tgcccgcgtc atcttcagcg 841 gctccctcga cttcttcagc gactccttct tcaactcagc agtgcagaag gcggcgcccg 901 gctcccagag gtattcccag acaggcaact atgaactagc tgtggccctc tcccgctggg 961 tgttcaagga ggagggtgTC CTCCGTGTGG GGCCTGTGTC CCATCATCGG GTGGGTGAGA 1021 CAGCCCCACC CAATGCCTAC ACTGTCACTG ACCTAGTGGA GTATAGCATC GTGATCCAGC 1081 AGCTCTCAAA TGGCAAATGG GTCCCCTTTG ATGGCGATGA CATTCAGCTG GAGTTTGTCC 1141 GCATTGATCC TTTTGTGAGG ACCTTCCTGA AGAAGAAAGG TGGCAAATAC AGTGTTCAGT 1201 TCAAGTTGCC CGACGTGTAT GGTGTATTCC AGTTTAAAGT GGATTACAAC CGGCTAGGCT 1261 ACACACACCT GTACTCTTCC ACTCAGGTAT CCGTGCGGCC ACTCCAGCAC ACGCAGTATG 1321 AGCGCTTCAT CCCCTCGGCC TACCCCTACT ACGCCAGCGC CTTCTCCATG ATGCTGGGGC 1381 TCTTCATCTT CAGCATCGTC TTCTTGCACA TGAAGGAGAA GGAGAAGTCC GACTGCCCAA 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAA CCAACACTTT AAGAAACATT CGCACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att