Construct: ORF TRCN0000471357
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001600.1_s317c1
- Derived from:
- ccsbBroadEn_00816
- DNA Barcode:
- CATCCTTATAACGCTGTCCATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDE (3416)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471357
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3416 | IDE | insulin degrading enzyme | NM_004969.4 | 100% | 100% | |
| 2 | human | 3416 | IDE | insulin degrading enzyme | NM_001322793.1 | 97.4% | 97.7% | (many diffs) |
| 3 | human | 3416 | IDE | insulin degrading enzyme | NM_001322794.1 | 96.1% | 96.1% | 659_660ins117 |
| 4 | human | 3416 | IDE | insulin degrading enzyme | NM_001322795.1 | 95.9% | 95.9% | 0_1ins123 |
| 5 | human | 3416 | IDE | insulin degrading enzyme | NM_001322796.1 | 95.9% | 95.9% | 0_1ins123 |
| 6 | human | 3416 | IDE | insulin degrading enzyme | XM_017016187.1 | 95.9% | 95.9% | 0_1ins123 |
| 7 | human | 3416 | IDE | insulin degrading enzyme | XM_017016188.1 | 95.9% | 95.9% | 0_1ins123 |
| 8 | human | 3416 | IDE | insulin degrading enzyme | XM_017016189.1 | 95.9% | 95.9% | 0_1ins123 |
| 9 | human | 3416 | IDE | insulin degrading enzyme | XM_017016190.1 | 93.4% | 93.7% | (many diffs) |
| 10 | human | 3416 | IDE | insulin degrading enzyme | XR_945727.3 | 67% | 1_74del;1958_2102del;2414_2415ins862 | |
| 11 | human | 3416 | IDE | insulin degrading enzyme | XR_001747103.2 | 64.5% | (many diffs) | |
| 12 | human | 3416 | IDE | insulin degrading enzyme | NR_136399.1 | 50.7% | 1_76del;1816_1939del;3258_6020del | |
| 13 | human | 3416 | IDE | insulin degrading enzyme | NM_001165946.1 | 45.5% | 45.5% | 0_1ins1665 |
| 14 | human | 3416 | IDE | insulin degrading enzyme | NM_001322797.1 | 43.4% | 43.2% | (many diffs) |
| 15 | mouse | 15925 | Ide | insulin degrading enzyme | NM_031156.3 | 89% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3126
- ORF length:
- 3057
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggtaccgg ctagcgtggc ttctgcaccc cgcactgccc agcaccttcc 121 gctcagtcct cggcgcccgc ctgccgcctc cggagcgcct gtgtggtttc caaaaaaaga 181 cttacagcaa aatgaataat ccagccatca agagaatagg aaatcacatt accaagtctc 241 ctgaagacaa gcgagaatat cgagggctag agctggccaa tggtatcaaa gtacttctta 301 tcagtgatcc caccacggat aagtcatcag cagcacttga tgtgcacata ggttcattgt 361 cggatcctcc aaatattgct ggcttaagtc atttttgtga acatatgctt tttttgggaa 421 caaagaaata ccctaaagaa aatgaataca gccagtttct cagtgagcat gcaggaagtt 481 caaatgcctt tactagtgga gagcatacca attactattt tgatgtttct catgaacacc 541 tagaaggtgc cctagacagg tttgcacagt tttttctgtg ccccttgttc gatgaaagtt 601 gcaaagacag agaggtgaat gcagttgatt cagaacatga gaagaatgtg atgaatgatg 661 cctggagact ctttcaattg gaaaaagcta cagggaatcc taaacacccc ttcagtaaat 721 ttgggacagg taacaaatat actctggaga ctagaccaaa ccaagaaggc attgatgtaa 781 gacaagagct actgaaattc cattctgctt actattcatc caacttaatg gctgtttgtg 841 ttttaggtcg agaatcttta gatgacttga ctaatctggt ggtaaagtta ttttctgaag 901 tagagaacaa aaatgttcca ttgccagaat ttcctgaaca ccctttccaa gaagaacatc 961 ttaaacaact ttacaaaata gtacccatta aagatattag gaatctctat gtgacatttc 1021 ccatacctga ccttcagaaa tactacaaat caaatcctgg tcattatctt ggtcatctca 1081 ttgggcatga aggtcctgga agtctgttat cagaacttaa gtcaaagggc tgggttaata 1141 ctcttgttgg tgggcagaag gaaggagccc gaggttttat gttttttatc attaatgtgg 1201 acttgaccga ggaaggatta ttacatgttg aagatataat tttgcacatg tttcaataca 1261 ttcagaagtt acgtgcagaa ggacctcaag aatgggtttt ccaagagtgc aaggacttga 1321 atgctgttgc ttttaggttt aaagacaaag agaggccacg gggctataca tctaagattg 1381 caggaatatt gcattattat cccctagaag aggtgctcac agcggaatat ttactggaag 1441 aatttagacc tgacttaata gagatggttc tcgataaact cagaccagaa aatgtccggg 1501 ttgccatagt ttctaaatct tttgaaggaa aaactgatcg cacagaagag tggtatggaa 1561 cccagtacaa acaagaagct ataccggatg aagtcatcaa gaaatggcaa aatgctgacc 1621 tgaatgggaa atttaaactt cctacaaaga atgaatttat tcctacgaat tttgagattt 1681 taccgttaga aaaagaggcg acaccatacc ctgctcttat taaggataca gctatgagca 1741 aactttggtt caaacaagat gataagtttt ttttgccgaa ggcttgtctc aactttgaat 1801 ttttcagccc atttgcttat gtggacccct tgcactgtaa catggcctat ttgtaccttg 1861 agctcctcaa agactcactc aacgagtatg catatgcagc agagctagca ggcttgagct 1921 atgatctcca aaataccatc tatgggatgt atctttcagt gaaaggttac aatgacaagc 1981 agccaatttt actaaagaag attattgaga aaatggctac ctttgagatt gatgaaaaaa 2041 gatttgaaat tatcaaagaa gcatatatgc gatctcttaa caatttccgg gctgaacagc 2101 ctcaccagca tgccatgtac tacctccgct tgctgatgac tgaagtggcc tggactaaag 2161 atgagttaaa agaagctctg gatgatgtaa cccttcctcg ccttaaggcc ttcatacctc 2221 agctcctgtc acggctgcac attgaagccc ttctccatgg aaacataaca aagcaggctg 2281 cattaggaat tatgcagatg gttgaagaca ccctcattga acatgctcat accaaacctc 2341 tccttccaag tcagctggtt cggtatagag aagttcagct ccctgacaga ggatggtttg 2401 tttatcagca gagaaatgaa gttcacaata actgtggcat cgagatatac taccaaacag 2461 acatgcaaag cacctcagag aatatgtttc tggagctctt ctgtcagatt atctcggaac 2521 cttgcttcaa caccctgcgc accaaggagc agttgggcta tatcgtcttc agcgggccac 2581 gtcgagctaa tggcatacag ggcttgagat tcatcatcca gtcagaaaag ccacctcact 2641 acctagaaag cagagtggaa gctttcttaa ttaccatGGA AAAGTCCATA GAGGACATGA 2701 CAGAAGAGGC CTTCCAAAAA CACATTCAGG CATTAGCAAT TCGTCGACTA GACAAACCAA 2761 AGAAGCTATC TGCTGAGTGT GCTAAATACT GGGGAGAAAT CATCTCCCAG CAATATAATT 2821 TTGACAGAGA TAACACTGAG GTTGCATATT TAAAGACACT TACCAAGGAA GATATCATCA 2881 AATTCTACAA GGAAATGTTG GCAGTAGATG CTCCAAGGAG ACATAAGGTA TCCGTCCATG 2941 TTCTTGCCAG GGAAATGGAT TCTTGTCCTG TTGTTGGAGA GTTCCCATGT CAAAATGACA 3001 TAAATTTGTC ACAAGCACCA GCCTTGCCAC AACCTGAAGT GATTCAGAAC ATGACCGAAT 3061 TCAAGCGTGG TCTGCCACTG TTTCCCCTTG TGAAACCACA TATTAACTTC ATGGCTGCAA 3121 AACTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3181 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3241 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACATCCTTAT AACGCTGTCC ATTCAACGCG 3301 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt