Construct: ORF TRCN0000471372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008138.1_s317c1
- Derived from:
- ccsbBroadEn_09071
- DNA Barcode:
- CAAACGTAGTTTTACTCTATAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INTS14 (81556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81556 | INTS14 | integrator complex subunit 14 | NM_001366360.2 | 99.8% | 99.8% | 14T>G;1236G>A |
| 2 | human | 81556 | INTS14 | integrator complex subunit 14 | NM_001207058.3 | 90.2% | 89.9% | (many diffs) |
| 3 | human | 81556 | INTS14 | integrator complex subunit 14 | NM_001136043.3 | 84.6% | 84.7% | 0_1ins237;999G>A |
| 4 | human | 81556 | INTS14 | integrator complex subunit 14 | NM_001207059.3 | 84.6% | 84.7% | 0_1ins237;999G>A |
| 5 | human | 81556 | INTS14 | integrator complex subunit 14 | NM_001366364.2 | 84.6% | 84.7% | 0_1ins237;999G>A |
| 6 | human | 81556 | INTS14 | integrator complex subunit 14 | NM_001366365.2 | 84.6% | 84.7% | 0_1ins237;999G>A |
| 7 | human | 81556 | INTS14 | integrator complex subunit 14 | XM_006720711.2 | 84.6% | 84.7% | 0_1ins237;999G>A |
| 8 | human | 81556 | INTS14 | integrator complex subunit 14 | XM_011522101.2 | 84.6% | 84.7% | 0_1ins237;999G>A |
| 9 | human | 81556 | INTS14 | integrator complex subunit 14 | NR_045104.2 | 67.9% | (many diffs) | |
| 10 | human | 81556 | INTS14 | integrator complex subunit 14 | NR_045901.2 | 63.2% | (many diffs) | |
| 11 | human | 81556 | INTS14 | integrator complex subunit 14 | NR_045106.3 | 60% | (many diffs) | |
| 12 | human | 81556 | INTS14 | integrator complex subunit 14 | NR_045105.3 | 57.9% | (many diffs) | |
| 13 | mouse | 69882 | Ints14 | integrator complex subunit 14 | NM_001077631.2 | 90% | 97.4% | (many diffs) |
| 14 | mouse | 69882 | Ints14 | integrator complex subunit 14 | NM_175153.4 | 90% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1620
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gacagtgggg gtaatggatg tatccctttc catgacccga cctgtgtcta 121 ttgaggggtc cgaggaatac cagcgtaagc acctagcagc ccatggttta acgatgctgt 181 ttgagcacat ggccacaaat tacaagcttg aatttacagc acttgtggtt ttttcatcac 241 tttgggagtt gatggtcccc ttcacgagag attataatac cctacaggaa gcactaagta 301 atatggatga ttatgacaaa acctgcttgg agtctgcatt agttggtgtt tgcaatatcg 361 ttcagcaaga atggggtggt gcaattcctt gccaggttgt cctggtgaca gacggctgtc 421 ttggcattgg tagagggtca ctgcgacatt ccctagccac tcaaaatcaa cgaagtgaga 481 gcaacaggtt tccactacct tttcctttcc catctaagtt atatatcatg tgcatggcga 541 atttggagga gctccagagc accgattcct tggaatgcct tgaacgtctc atagatttaa 601 acaatggtga agggcagatt tttactattg atggccccct gtgcttgaag aatgtacagt 661 ctatgtttgg aaaactgata gatttggcat atacgccttt ccatgctgtt ctcaagtgtg 721 gccacctaac tgctgatgta caagtcttcc ccaggccaga accttttgtt gtagatgaag 781 aaattgatcc tatccctaaa gtcattaaca cagatttgga aatagtggga tttattgata 841 tagctgatat ttcaagtccc ccagttctgt ccagacatct ggtcttacct atagcactta 901 acaaagaagg tgatgaggtg ggtactggca tcactgatga caatgaagat gaaaattcag 961 ccaatcagat tgcaggcaaa atacccaact tttgtgtcct gctccatggt agcctaaaag 1021 tggaaggaat ggtagcgatt gttcaattag gtcctgaatg gcatggaatg ctctactccc 1081 aagctgacag caagaagaaa tcaaacctca tgatgtctct ctttgagcct ggcccagaac 1141 ctctcccatg gctagggaaa atggcacagt tgggtccTAT TTCAGATGCT AAAGAAAACC 1201 CTTATGGCGA GGATGACAAT AAGAGTCCAT TCCCCCTGCA GCCCAAAAAC AAACGCAGTT 1261 ATGCCCAGAA TGTGACTGTC TGGATCAAAC CCAGCGGCCT ACAGACAGAT GTACAGAAGA 1321 TTTTAAGAAA TGCAAGGAAA CTACCTGAAA AAACACAGAC ATTCTATAAG GAGCTGAACC 1381 GTTTGCGAAA GGCCGCTCTA GCCTTTGGTT TCCTGGACCT GCTGAAAGGG GTGGCTGACA 1441 TGCTGGAAAG GGAATGCACA CTGCTGCCTG AGACAGCCCA CCCTGATGCT GCATTCCAGC 1501 TGACCCATGC TGCCCAGCAG CTCAAGCTGG CCAGTACCGG CACCTCTGAG TATGCCGCTT 1561 ATGACCAGAA CATCACACCT TTGCACACGG ACTTCTCTGG GAGCAGCACT GAAAGAATTT 1621 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1681 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1741 TTTATATATC TTGTGGAAAG GACGACAAAC GTAGTTTTAC TCTATAGTTA CGCGTTAAGT 1801 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt