Construct: ORF TRCN0000471382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012434.1_s317c1
- Derived from:
- ccsbBroadEn_09858
- DNA Barcode:
- GAATTTGAAATTTATAAATTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDIAS (220042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471382
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220042 | DDIAS | DNA damage induced apoptosi... | NM_001363481.2 | 99.8% | 99.6% | 650T>C;1380G>T;2524G>A |
| 2 | human | 220042 | DDIAS | DNA damage induced apoptosi... | NM_145018.4 | 99.8% | 99.6% | 650T>C;1380G>T;2524G>A |
| 3 | human | 220042 | DDIAS | DNA damage induced apoptosi... | XM_011544836.2 | 99.8% | 99.6% | 650T>C;1380G>T;2524G>A |
| 4 | human | 220042 | DDIAS | DNA damage induced apoptosi... | XM_024448400.1 | 99.8% | 99.6% | 650T>C;1380G>T;2524G>A |
| 5 | human | 220042 | DDIAS | DNA damage induced apoptosi... | XM_011544837.1 | 69.7% | 69.6% | 0_1ins903;477G>T;1621G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3060
- ORF length:
- 2994
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cagaagacga aaatttcttc tagcctcagt acttgctctc cagaattcaa 121 gttttatata tccatcatgt cagaagtgct tctctaggat aatcctggtc tccaaaaggt 181 ctaattgtcc aaaatgtggc tctactggtg aatctggaaa tgccaattac agatacaaac 241 tttccttaaa agttgcagaa tcaaacaaat tgtttgttat tactgtattt ggaagttgct 301 tagatacatt ttttggtctt actgccactg gtttgcacag gtacattcag gatcctaata 361 aaattccaga aacactggac aatgatacaa ctcagaatct attaactaaa gcagttgaaa 421 cttgctttgt tggacaaagc tttatttttg gagtgacgaa ttttgaaaac caacctggac 481 aaggttcaga tgccagtaac ttcttacagc aatgctctga ccacaaaaga aaagccaaag 541 cactagtggc ttgccagatt gttctaccag acccaggtat tgcaggcttt actgtcattg 601 actacttcca tcaacttttg cagactttta atttcaggaa acttcagtgt gactctcagg 661 cacctaacaa tcacttactt gctttagatc actcaaatag tgatctcagc agcacatata 721 cttctgacag cacttctgat tttttcaagt cctgcagcaa ggatactttt tcaaaattct 781 ggcagccatc acttgaattc acttgcattg tttcacaact aacagataat gatgattttt 841 cagcttcaga acaaagtaag gcctttggta ctcttcagca gaacagaaag tccatctcca 901 ttgcagaggc cactggttcc agtagctgcc atgatcccat tcaggattca tggagccttg 961 tttcatatat ggataaaaag agtacagcag aaaagttggg taaagaactt ggcttacaag 1021 ctaaggagct gagtgcagtt cacagcagtc atcatgaaat tggagttaat gactctaatt 1081 tattctcttt ggaaatgcga gagccccttg agtcaagtaa tacaaaatcc ttccacagtg 1141 cagtggaaat taaaaatagg tcccagcatg agctaccatg ttttcagcat catggtatag 1201 ataccccaac tagccttcag aagagatctg catgttgtcc accttcgtta ctcagacttg 1261 aagagacagc cagcagttcc caggatggtg accctcaaat ttgggatgat ctgccattct 1321 ctgaaagcct gaacaagttt ctggcagttc ttgaaagtga gattgctgta acccaggcag 1381 atgtcagtag taggaaacat catgtagata atgacattga taaatttcat gcagaccaca 1441 gcagtttatc tgtgactccc cagagaacta ctggagccct gcatacacca cctatagctt 1501 taagatcatc acaagtaata gtcaaagcaa actgtagcaa agatgacttc cttttcaact 1561 gtaaaggaaa tctaagtcct agtgttgaaa aggagtcaca accagataac aaagtagagg 1621 ctgtctctgt aaatcataat ggaagagata tgtcagaata ttttttaccg aatccttacc 1681 tgtcagctct gtcttcatct tcaaaagatt tagaaacaat agttactctt aagaagacta 1741 tcagaatctc accacacagg gagagtgacc attctagtct aaataacaaa tatttgaatg 1801 gatgtggaga aatatcagtt tcagaaatga atgaaaagtt gacaactctg tgttatagga 1861 agtataatga tgtctctgat ctttgcaaat tagaaaataa acaatattgt aggtggtcca 1921 agaaccaaga tgacagtttt acaatttgca ggaaacttac atatccttta gaaactcttt 1981 gcaatagtcc aaatagaagt acaaatacat tgaaagaaat gccttgggga catatcaata 2041 acaacgtaac acagagctat tctattggtt atgaaggtag ctatgatgcc tctgctgatc 2101 tctttgatga tattgctaaa gaaatggaca ttgcaactga gattaccaaa aaatcacagg 2161 atattttgtt aaaatgggga acatctttgg cagaaagtca cccttcagag tctgattttt 2221 cactgagatc actttctgaa gacttcatcc agccttcaca aaaattatcc ttgcaaagcc 2281 tatctgactc taggcattca agaacatgct ctccaacacc tcattttcaa tcagattcag 2341 aatataattt tgaaaatagt caagactttg ttccatgttc acagtcaact ccaatttcag 2401 ggttccacca aacaagaatt catgggataa acagagcttt caaaaaacct gtattttatt 2461 cagatcttga tggtaactat gaaaaaataa ggattttccc tgaaaatgac aaacagcaag 2521 ccagcccaag ctgtccaaaa aatataaaaa cacctagcca gaaaatcaga agccctattg 2581 tatctggtat ttcacaacca gacgttttca atcactaccc ttttgctgag tgccatgaaa 2641 ctgatagtga tgaatgggtc cctcctacca cacaaaaaat atttccttca gatatgcttg 2701 gattccaagg cataggtcta gggaaatgcc ttgctgccta tcatttccct gatcaacaag 2761 agttaccaag aaagaaactg aaacatatta gacaaggaac caataaaggt ttaattaaga 2821 agaaattaaa gaatatgctt gcagcagttg ttacgaaaaa gaaaactcat aaatataact 2881 gtaaaagttc aggctggatt tccaaatgtc cagacattca agtcttagca gcacctcagc 2941 tgcaccctat tcttggacct gattcttgtt cagaagtcaa atgttgcctt ccattttcag 3001 aaaaaggccc accttcagtg tgtGAAACTC GAAGTGCTTG GTCACCTGAA TTGTTTTCAT 3061 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3121 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3181 TTTATATATC TTGTGGAAAG GACGAGAATT TGAAATTTAT AAATTCGGGA CGCGTTAAGT 3241 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt