Construct: ORF TRCN0000471390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011647.1_s317c1
- Derived from:
- ccsbBroadEn_15012
- DNA Barcode:
- TTCTCCATATGTATTAAAACCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ICMT (23463)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23463 | ICMT | isoprenylcysteine carboxyl ... | NM_012405.4 | 100% | 100% | |
| 2 | human | 23463 | ICMT | isoprenylcysteine carboxyl ... | XM_011541140.2 | 66.1% | 66.1% | 0_1ins288 |
| 3 | mouse | 57295 | Icmt | isoprenylcysteine carboxyl ... | NM_133788.2 | 89% | 96.1% | (many diffs) |
| 4 | mouse | 57295 | Icmt | isoprenylcysteine carboxyl ... | NR_037908.1 | 14.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggctgcgcg gcgcgggctc cgccgggctc tgaggcgcgt ctcagcctcg 121 ccaccttcct gctgggcgcc tcggtgctcg cgctgccgct gctcacgcgc gccggcctgc 181 agggccgcac cgggctggcg ctctacgtgg ccgggctcaa cgcgctgctg ctgctgctct 241 atcggccgcc tcgctaccag atagccatcc gagcttgttt cctggggttt gtgttcggct 301 gcggcacgct gctaagtttt agccagtctt cttggagtca ctttggctgg tacatgtgct 361 ccctgtcatt gttccactat tctgaatact tggtgacagc agtcaataat cccaaaagtc 421 tgtccttgga ttcctttctc cTGAATCACA GCCTGGAGTA TACAGTAGCT GCTCTTTCTT 481 CTTGGTTAGA GTTCACACTT GAAAATATCT TTTGGCCAGA ACTGAAGCAG ATTACCTGGC 541 TCAGTGTCAC AGGGCTGCTG ATGGTGGTCT TCGGAGAATG TCTGAGGAAG GCGGCCATGT 601 TTACAGCTGG CTCCAATTTC AACCACGTGG TACAGAATGA AAAATCAGAT ACACATACTC 661 TGGTGACCAG TGGAGTGTAC GCTTGGTTTC GGCATCCTTC TTACGTCGGG TGGTTTTACT 721 GGAGTATTGG AACTCAGGTG ATGCTGTGTA ACCCCATCTG CGGCGTCAGC TATGCCCTGA 781 CAGTGTGGCG ATTCTTCCGC GATCGAACAG AAGAAGAAGA AATCTCACTA ATTCACTTTT 841 TTGGAGAGGA GTACCTGGAG TATAAGAAGA GGGTGCCCAC GGGCCTGCCT TTCATAAAGG 901 GGGTCAAGGT GGACCTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 961 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1021 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCTCCA TATGTATTAA 1081 AACCCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt