Construct: ORF TRCN0000471399
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005721.1_s317c1
- Derived from:
- ccsbBroadEn_13249
- DNA Barcode:
- TAACACATAACCTCGGTGGGTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1orf127 (148345)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471399
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148345 | C1orf127 | chromosome 1 open reading f... | NM_001366227.1 | 94.6% | 94.7% | (many diffs) |
| 2 | human | 148345 | C1orf127 | chromosome 1 open reading f... | XM_017000373.1 | 82.2% | 82.3% | (many diffs) |
| 3 | human | 148345 | C1orf127 | chromosome 1 open reading f... | NM_001170754.1 | 78.6% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2013
- ORF length:
- 1944
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagtgtccg atgctaaggt caaggctggg ccaggaaagc gtccactgtg 121 ggcccatgtt catccaggtc tcccggcccc tgcccctgtg gagggacaat agacagactc 181 catggctgct gtcccttcga ggggagctgg tggcttctct tgaagacgcc agcctgatgg 241 gactgtatgt ggacatgaat gccaccactg tcaccgtcca aagcccgaga caaggccttc 301 ttcagaggtg ggaggtgctg aacacctctg ctgagctcct gccactatgg ctggtgagcg 361 gtcaccatgc ctattcttta gaagctgctt gcccaccggt gtcattccag ccagagtcgg 421 aggtcttagt tcacatcccc aagcagagac tgggtctagt caaaagaggt tcctacattg 481 aggaaaccct gagcctcaga ttcctccgag tccaccagtc caacatcttt atggtgactg 541 agaacaagga ctttgtggtg gtcagcattc cggcggccgg ggtgctccag gtccagcgat 601 gccaagaagt cggaggaacc ccgggaacac aagctttcta tagggtagac ctgagcctgg 661 aatttgccga gatggctgcc ccggtcctct ggacagtgga gagcttcttc cagtgtgtgg 721 gttcaggaac agagtcgcct gcctcaactg ctgcactgag gaccactccc tccccaccat 781 ccccaggacc agagacccct ccagcgggag tgccacctgc tgcttcctcc cagaaggggc 841 ttggaccatt cctgcaaaca gccaaaccgg cgagaagagg ccagacatct gcctccattc 901 tccccagagt ggtgcaagct cagcgaggtc cccagcctcc cccaggggaa gcagggatcc 961 ctggacaccc cacacctcca gccacgctcc cctcggagcc tgtagagggt gtccaggcta 1021 gtccctggcg gccacgtcca gtcttgccaa cgcacccggc tctgaccctg cccgtgtcct 1081 cagatgcctc ctctccttca ccgccagccc cgaggcctga acgacctgaa tcacttctgg 1141 tctcaggacc atctgtcacc ctgactgaag gtctaggaac tgtgaggcct gaacaggacc 1201 ccgccaagtc tccaggaagt cccctcctgc tgagaggctt gtcaagcggg gatgtggctg 1261 cacctgagcc catcatgggg gagcccggcc aagccagtga ggagttccag ccattggcga 1321 ggccctggcg ggccacactg gctgcagagg agctggtttc tcaccgttct cccggagagc 1381 cccaggaaac gtgctctgga acggaggtgg agaggccacg ccagacaggg cctggtctcc 1441 ccagggaggg ggccaggggg cacatggacc tttcatcctc agaaccaagc caggacatag 1501 aggggccggg actctccatc ctgccagcga gggatgccac attctccacc ccaagtgtga 1561 ggcagccaga ccccagtgcc tggctgagtt caggacctga actcaccggg atgcccaggg 1621 tgaggctggc agcgcccctg gcagttcttc ctatggaacc TCTGCCACCA GAACCTGTTC 1681 GCCCAGCAGC TCTTCTGACA CCCGAAGCCT CATCTGTGGG AGGGCCAGAC CAGGCCCGAT 1741 ACCTGGAGTC AGCCCCTGGC TGGCCCGTGG GCCAGGAGGA GTGGGGGGTT GCACACACGT 1801 CCAGCCCTCC ATCCACGCAA ACCCTGAGCC TGTGGGCTCC CACAGGAGTG TTGCTACCCA 1861 GCCTGGTGGA GCTTGAATAC CCCTTCCAGG CTGGTCGGGG GGCCTCACTC CAGCAGGAGC 1921 TGACAGAGCC CACCTTGGCC CTCAGTGCTG AAAGCCACAG GCCTCCTGAG CTTCAAGACA 1981 GTGTGGAGGG GCTTTCTGAG AGGCCCTCAC GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2041 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2101 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 2161 ACACATAACC TCGGTGGGTA GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 2221 ttgtgaaaga tt