Construct: ORF TRCN0000471406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001621.1_s317c1
- Derived from:
- ccsbBroadEn_10359
- DNA Barcode:
- CCTTCATAAAGATATCAGTCACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECEL1P2 (347694)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471406
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 347694 | ECEL1P2 | endothelin converting enzym... | NR_028501.1 | 9.8% | 1_125del;255_1309del | |
| 2 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_001290787.2 | 4.9% | 4.6% | (many diffs) |
| 3 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_004826.4 | 4.9% | 4.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 243
- ORF end:
- 372
- ORF length:
- 129
- Sequence:
-
1 TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG 61 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 121 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 181 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 241 GCATGCGCGA GATCGAACGA CTGAACCCGC TGCTCATGCT GGAGGTCATC GAGGACTGCG 301 GGGGCTGGGA CCTGCGCGGC GCGGCGGAGC GCCGGGGGGT TGCTGCGCGA TGGGACCTCA 361 ACTGGCTGCT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 421 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 481 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT TCATAAAGAT ATCAGTCACT 541 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t