Construct: ORF TRCN0000471426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003962.1_s317c1
- Derived from:
- ccsbBroadEn_10741
- DNA Barcode:
- AGATATAACTATTCGACCGTACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL8A2 (1296)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471426
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1296 | COL8A2 | collagen type VIII alpha 2 ... | NM_005202.4 | 60.7% | 60.7% | 769_1596del |
| 2 | human | 1296 | COL8A2 | collagen type VIII alpha 2 ... | XM_005270477.3 | 54.7% | 54.7% | 1_231del;1000_1827del |
| 3 | human | 1296 | COL8A2 | collagen type VIII alpha 2 ... | NM_001294347.2 | 51.4% | 51.4% | 0_1ins195;574_1401del |
| 4 | mouse | 329941 | Col8a2 | collagen, type VIII, alpha 2 | NM_199473.2 | 53.2% | 57.6% | (many diffs) |
| 5 | mouse | 329941 | Col8a2 | collagen, type VIII, alpha 2 | XM_017320266.1 | 52% | 56.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1350
- ORF length:
- 1281
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggggact ctgacacccc tgtcttcgct gctgctgctg ctactggtgc 121 tggtgctggg gtgtgggccg cgggcgtcct ctggtggcgg ggccggtggg gcggcgggct 181 atgccccagt gaagtacatc cagcccatgc agaaaggacc tgtgggaccg cccttccgtg 241 agggcaaagg ccagtacctg gaaatgcctc taccgctgct gccgatggac ctgaagggag 301 agcccggccc ccctgggaag cccgggcctc ggggtccccc tggcccccct ggcttcccag 361 gaaaaccagg catgggaaag ccaggactcc atgggcagcc tggccctgct gggccccctg 421 gcttctcccg gatgggcaag gctggtcccc cagggctccc tggcaaggtc gggccaccag 481 ggcagccggg gcttcggggg gagccaggaa tacgagggga ccagggcctc cggggacccc 541 caggaccccc tggcctcccg ggcccctcag gcattactat ccctggaaaa ccaggtgccc 601 aaggggtgcc agggccccca ggattccagg gggaaccagg gccccagggg gagcctgggc 661 ccccaggtga tcgaggcctc aagggggata atggagtggg ccagcccggg ctgcctgggg 721 ccccagggca ggggggtgcc cccggccccc ccggcctccc tggtccagct ggcttaggca 781 aacctggttt ggatgggctt cctggggccc caggagacaa gggtgagtct gggcctcctg 841 gggccttcga tgagactggc atcgcaggct tgcacctgcc caacggcggt gtggagggtg 901 ccgtgctggg caaggggggC AAGCCACAGT TTGGGCTGGG CGAGCTGTCT GCCCATGCCA 961 CACCGGCCTT CACTGCGGTG CTCACCTCGC CCTTCCCCGC CTCGGGCATG CCCGTGAAAT 1021 TTGACCGGAC TCTCTACAAT GGCCACAGCG GCTACAACCC AGCCACTGGC ATCTTCACCT 1081 GCCCTGTGGG CGGCGTCTAC TACTTTGCTT ACCATGTGCA CGTCAAGGGC ACCAACGTGT 1141 GGGTGGCCCT GTACAAGAAC AACGTGCCGG CCACCTATAC CTACGATGAG TACAAGAAGG 1201 GCTACCTGGA CCAGGCATCT GGTGGGGCCG TGCTCCAGCT GCGGCCCAAC GACCAGGTCT 1261 GGGTGCAGAT GCCGTCGGAC CAGGCCAACG GCCTCTACTC CACGGAGTAC ATCCACTCCT 1321 CCTTTTCAGG ATTCTTGCTC TGCCCCACAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGATA 1501 TAACTATTCG ACCGTACTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt