Transcript: Human NM_001294347.2

Homo sapiens collagen type VIII alpha 2 chain (COL8A2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
COL8A2 (1296)
Length:
4524
CDS:
278..2194

Additional Resources:

NCBI RefSeq record:
NM_001294347.2
NBCI Gene record:
COL8A2 (1296)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001294347.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000371819 TCGGGCATGCCCGTGAAATTT pLKO_005 1844 CDS 100% 15.000 21.000 N COL8A2 n/a
2 TRCN0000083090 CACCTATACCTACGATGAGTA pLKO.1 2014 CDS 100% 4.950 6.930 N COL8A2 n/a
3 TRCN0000083091 CCGGCCACCTATACCTACGAT pLKO.1 2009 CDS 100% 1.000 1.400 N COL8A2 n/a
4 TRCN0000371820 CATGCAGGCTGAGATTGTTTC pLKO_005 2345 3UTR 100% 10.800 7.560 N COL8A2 n/a
5 TRCN0000083088 GCCCACAACTTCTCCAAACAA pLKO.1 3796 3UTR 100% 5.625 3.938 N COL8A2 n/a
6 TRCN0000371878 TTGGCCCGAGGAGACTAACTT pLKO_005 2312 3UTR 100% 5.625 3.938 N COL8A2 n/a
7 TRCN0000083089 AGGCATTACTATCCCTGGAAA pLKO.1 583 CDS 100% 4.950 3.465 N COL8A2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001294347.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10741 pDONR223 100% 51.4% 51.4% None 0_1ins195;574_1401del n/a
2 ccsbBroad304_10741 pLX_304 0% 51.4% 51.4% V5 0_1ins195;574_1401del n/a
3 TRCN0000471426 AGATATAACTATTCGACCGTACTC pLX_317 33.8% 51.4% 51.4% V5 0_1ins195;574_1401del n/a
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