Construct: ORF TRCN0000471438
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002796.1_s317c1
- Derived from:
- ccsbBroadEn_01450
- DNA Barcode:
- ATCTAGGGTCGCGATTGTGGTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS10 (6204)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471438
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6204 | RPS10 | ribosomal protein S10 | NM_001014.5 | 100% | 100% | |
| 2 | human | 6204 | RPS10 | ribosomal protein S10 | NM_001203245.3 | 100% | 100% | |
| 3 | human | 6204 | RPS10 | ribosomal protein S10 | NM_001204091.2 | 100% | 100% | |
| 4 | human | 376693 | RPS10P7 | ribosomal protein S10 pseud... | NR_026667.1 | 66.9% | (many diffs) | |
| 5 | human | 100529239 | RPS10-NUDT3 | RPS10-NUDT3 readthrough | NM_001202470.2 | 54.2% | 52.7% | (many diffs) |
| 6 | mouse | 67097 | Rps10 | ribosomal protein S10 | NM_025963.3 | 91.1% | 98.7% | (many diffs) |
| 7 | mouse | 67097 | Rps10 | ribosomal protein S10 | XM_011246579.2 | 67.7% | 73.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gatgccTAAG AAGAACCGGA TTGCCATTTA TGAACTCCTT TTTAAGGAGG 121 GAGTCATGGT GGCCAAGAAG GATGTCCACA TGCCTAAGCA CCCGGAGCTG GCAGACAAGA 181 ATGTGCCCAA CCTTCATGTC ATGAAGGCCA TGCAGTCTCT CAAGTCCCGA GGCTACGTGA 241 AGGAACAGTT TGCCTGGAGA CATTTCTACT GGTACCTTAC CAATGAGGGT ATCCAGTATC 301 TCCGTGATTA CCTTCATCTG CCCCCGGAGA TTGTGCCTGC CACCCTACGC CGTAGCCGTC 361 CAGAGACTGG CAGGCCTCGG CCTAAAGGTC TGGAGGGTGA GCGACCTGCG AGACTCACAA 421 GAGGGGAAGC TGACAGAGAT ACCTACAGAC GGAGTGCTGT GCCACCTGGT GCCGACAAGA 481 AAGCCGAGGC TGGGGCTGGG TCAGCAACCG AATTCCAGTT TAGAGGCGGA TTTGGTCGTG 541 GACGTGGTCA GCCACCTCAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATCT AGGGTCGCGA 721 TTGTGGTTTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt