Construct: ORF TRCN0000471440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013239.1_s317c1
- Derived from:
- ccsbBroadEn_04361
- DNA Barcode:
- AGCTGACAGACCGTGTCGAGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR83 (84292)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471440
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84292 | WDR83 | WD repeat domain 83 | NM_001099737.3 | 100% | 100% | |
| 2 | human | 84292 | WDR83 | WD repeat domain 83 | NM_032332.4 | 100% | 100% | |
| 3 | human | 84292 | WDR83 | WD repeat domain 83 | NR_029375.2 | 64% | 1_342del;548_549insGGTGCTGGATGCGGCCG;1271_1433del | |
| 4 | mouse | 67836 | Wdr83 | WD repeat domain containing 83 | NM_026399.2 | 88% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttccctgag ccaaagccgc ggcctccaga gctgccgcag aaacggttga 121 agacgctgga ctgcgggcag ggggcagtgc gagccgtacg atttaatgtg gatggcaatt 181 actgcctgac gtgcggcagt gacaagacgc tgaagctgtg gaacccgctt cgggggacgc 241 tgctgcggac gtacagcggc cacggctacg aggtgctgga tgcggccggc tcctttgaca 301 acagtagtct ctgctccggc ggcggggaca aggcggtggt tctgtgggat gtggcatcag 361 ggcaggtcgt gcgcaaattc cggggccacg cagggaaggt gaacacggtg cagtttaatg 421 aagaggccac agttatcctg tccggctcta ttgattccag tatccgctgt tgggattgcc 481 gctcacggag gcctgagcca gtgcagacgc tggatgaggc cagagatggc gtgtccagtg 541 tgaaggtgtc agaccacgag atcctggcag gctccgtgga tggccgcgtg agacgctatg 601 acctaaggat ggggcagctc ttcTCAGACT ACGTGGGCAG CCCCATCACC TGCACCTGCT 661 TCAGCCGGGA TGGGCAGTGC ACCCTGGTGT CCAGCCTGGA CTCCACATTG CGGCTCCTGG 721 ACAAAGACAC AGGGGAGCTG CTGGGCGAGT ACAAGGGCCA TAAGAACCAG GAATACAAGC 781 TGGACTGCTG CCTGAGCGAG CGTGACACAC ATGTGGTCAG CTGTTCTGAG GACGGGAAGG 841 TGTTCTTCTG GGACCTGGTG GAGGGTGCGC TGGCTCTGGC CCTGCCTGTG GGTTCCGGTG 901 TGGTGCAGTC GCTGGCCTAC CACCCAACAG AGCCCTGCCT GCTGACCGCC ATGGGAGGCA 961 GCGTCCAGTG CTGGCGAGAG GAGGCCTATG AGGCAGAGGA TGGAGCAGGC TGCCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAAGCT GACAGACCGT GTCGAGTCTT ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt